Journal ArticleDOI
Cysteine oxidation and annulment of amphotericin B inhibition of Coccidioides immitis
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TLDR
Evidence is presented that exogeneous cysteine is oxidized by endospores only after Amphotericin damages cell barriers, and it is speculated that the resulting oxidation is an important feature in the annulment of amphotericin activity.Abstract:
The capacity of cysteine to annul the inhibitory action of amphotericin B on Coccidioides immitis was studied. Concomitant with inhibited growth were suppressed respiration patterns. Added cysteine, however, which was not oxidized by untreated endospores, was attacked by amphotericin-treated cells; it was oxidized to cystine. A crude cysteine oxidase was obtained from endospores that had not been exposed to cysteine. Evidence is presented that exogeneous cysteine is oxidized by endospores only after amphotericin damages cell barriers. It is speculated that the resulting oxidation is an important feature in the annulment of amphotericin activity.read more
Citations
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Delayed Hypersensitivity Responses ofExperimental Animals toHistoplasmin fromtheYeastandMycelial Phases of Histoplasma capsulatum
TL;DR: CYL reagents were nonreactive in guinea pigs infected with Coccidioides immitis, whereas the commercial Food and Drug Administration preparations cross-reacted to some degree, and inter-lot biological variation was minimal.
Journal ArticleDOI
Effects of ergosterol, palmitic acid and related simple lipids on the recovery of Candida albicans from ultraviolet irradiation
Alvin Sarachek,N. P. Higgins +1 more
TL;DR: The behaviors of cells damaged by uv and by amphotericin B, a membrane specific fungicidal antibiotic, suggest that lipids remediate an uv-induced derangement of the structure of the cell membrane critical for the initiation of cell division.
Journal ArticleDOI
Properties and assay of spherulins from Coccidioides immitis in delayed sensitivity responses of animals.
TL;DR: The properties of spherule phase coccidioidin (spherulin) in dermal sensitivity reactions were studied with regard to strain source, assay variables, potency and stability during storage, and the exceptional spherulin showed low activity and had lower protein, polysaccharide and nitrogen contents than the other reagents.
Journal ArticleDOI
Inactivation of amphotericin B by reducing agents: influences on growth inhibition of Candida albicans and lysis of erythrocytes.
M.R. Weis,H. B. Levine +1 more
TL;DR: Cysteine-HCl, sodium sulfite, sodium dithionite, and sodium thiosulfate annulled amphotericin-mediated growth inhibition of susceptible organisms and protected mouse erthrocytes from amphotericIn-induced hemolysis.
Effect of Glucose and Phosphate on Growth of the Dermatophyte Trichophyton Mentagrophytes
TL;DR: Thesis as mentioned in this paper is brought to you for free and open access by the Theses and Dissertations at Loyola eCommons and has been accepted for inclusion in Master's Theses by an authorized administrator.
References
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Journal ArticleDOI
Symposium on biochemical bases of morphogenesis in fungi. iv. molecular bases of form in yeasts.
Journal ArticleDOI
Action of the polyene antibiotics filipin, nystatin and n-acetylcandidin on the yeast cell membrane.
TL;DR: It is suggested that the polyenes present a full spectrum of effects which relate to the degree of physical damage to the cell membrane, which range from filipin which destroys general structural integrity of the membrane even at the minimum concentration necessary to inhibit glycolysis to n-acetylcandidin which produces only minimal damage so that relatively specific defects are observed.
Journal ArticleDOI
Spherule coccidioidin in delayed dermal sensitivity reactions of experimental animals
TL;DR: A new coccidioidin, derived from the spherule phase of Coccidioides immitis cultured in vitro, was examined for its capacity to elicit delayed dermal hypersensitivity reactions in mice and guinea pigs.
Journal ArticleDOI
The action of amphotericin b on mycoplasma laidlawii.
TL;DR: M. laidlawii grown in cholesterol-free medium are resistant to amphotericin B; when grown in the presence of cholesterol they are sensitive to the drug, and this change is associated with loss of some of the radioactivity previously incorporated from labeled cholesterol.
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