Journal ArticleDOI
Cystic Fibrosis: Characterization of the Inhibitor to Ciliary Action in Oyster Gills
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The inhibitor to oyster ciliary activity was isolated from serum of cystic fibrosis patients and heterozygotes and eluted with the immunoglobulin G fraction, which in normal individuals did not inhibit Oyster cillary activity.Abstract:
The inhibitor to oyster ciliary activity was isolated from serumof cystic fibrosis patients and heterozygotes.The inhibitinig protein fraction wast a cation as judged by electrophoresis; it had a molecuer weight of 125,000 to 200,000 as judged by gel filtration; antd oil diethylaminoethyl-cellulose chromatography it eluted with the immunoglobulin G fraction. The analogous fraction in normal individuals did not inhibit oyster cillary activity.read more
Citations
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Book ChapterDOI
Biological activities of immunoglobulins of different classes and subclasses.
TL;DR: It has now been shown that the antibodies of the various classes and subclasses differ in their biological activities, and, in the future, one should attempt to determine how frequently restricted immune responses occur and how important such restricted antibody formation is in immune deficiency syndromes.
Journal ArticleDOI
Usefulness of serum IgG4 in the diagnosis and follow-up of autoimmune pancreatitis: a systematic literature review and meta-analysis
TL;DR: The heterogeneity of the studies published until now means that more studies are necessary in order to better evaluate the true accuracy of IgG4 in discriminating AIP versus other autoimmune diseases, and its determination should be included in the diagnostic workup of this disease.
Journal ArticleDOI
Cystic fibrosis: a not-so-fatal disease.
TL;DR: Cystic fibrosis is an intricate disease involving the whole patient and requiring complicated treatment, and attention to nutrition is mandatory.
Journal ArticleDOI
Studies on cystic fibrosis using isoelectric focusing. I. An assay for detection of cystic fibrosis homozygotes and heterozygote carriers from serum.
TL;DR: Analysis of serum samples from 68 patients with a variety of other diseases indicated that the C/F factor protein described in this study appears to be diagnostic for C/f genotypes, with the possible exception of patients with certain types of leukemia.
Journal ArticleDOI
Demonstration of serum protein differences in cystic fibrosis by isoelectric focusing in thin-layer polyacrylamide gels.
TL;DR: Constant serum protein differences between the three serum types have been demonstrated using isoelectric focusing in thin-layer polyacrylamide gels and the concentration of both proteins was demonstrated to be variable among the heterozygotes as shown by electrofocusing of different volumes of serum.
References
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Journal ArticleDOI
Estimation of the molecular weights of proteins by Sephadex gel-filtration.
TL;DR: The results are similar to those of previous studies, where the objective was to establish a cause-and-effect relationship, rather than a straightforward relationship between the number of cells and the content of the molecule.
Journal ArticleDOI
Abnormal Serum Factor in Patients with Cystic Fibrosis of the Pancreas
TL;DR: Serum from patients with cystic fibrosis of the pancreas (CFP) was found to disorganize the ciliary rhythm in explants of respiratory epithelium and the macromolecules in the serum may alter transport of electrolytes, thereby producing changes in the action potential within the cell and resulting in a disorganized ciliary beat.
Journal ArticleDOI
Chromatography of proteins on cellulose ion-exchangers.
Journal ArticleDOI
Oyster Ciliary Inhibition by Cystic Fibrosis Factor
TL;DR: Seriums from 47 patients With cystic fibrosis antd 19 heterozygotes caused ciliary cessation within 35 minutes, whereas serums from only 2 of 64 individuals without cystic Fibrosis inhibited ciliary activity within this time.
Journal ArticleDOI
Inhibition of the motility of gill cilia of Dreissensia by plasma of cystic fibrosis patients and their parents.
TL;DR: Observations using segments of gills of the fresh-water mussel Dreissensia as an experimental system are described, which showed that whole sera and a euglobulin component isolated from the sera of patients with cystic fibrosis disrupted the normal beat of the cilia of human and rabbit respiratory tract epithelium in tissue culture.