Journal ArticleDOI
Cytogenetic distinction between the TK+ and TK− chromosomes in the L5178Y TK+ / − 3.7.2C mouse-lymphoma cell line
TLDR
A centromeric heteromorphism in the chromosomes 11 is discovered which, together with the types of chromosome rearrangements thus far catalogued for TK+ / − → TK− / − mutagenesis, allows us to propose a cytogenetic distinction between the TK-competent (TK+) and Tk-deficient (Tk−) chromosome.About:
This article is published in Mutation Research Letters.The article was published on 1982-12-01. It has received 69 citations till now. The article focuses on the topics: Karyotype.read more
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Journal ArticleDOI
Analysis of trifluorothymidine-resistant (TFTr) mutants of L5178Y/TK+/- mouse lymphoma cells.
Martha M. Moore,Donald Clive,John C. Hozier,Barry E. Howard,A. Gail Batson,Nancy T. Turner,Jeffrey Sawyer +6 more
TL;DR: The inclusion of sigma mutants in the total induced mutant frequency, as well as distinguishing them as a separate subpopulation of TK-deficient mutants, is essential in obtaining maximum utility of the information provided by the L5178Y/TK+/- mouse lymphoma assay.
Journal ArticleDOI
ICH-harmonised guidances on genotoxicity testing of pharmaceuticals: evolution, reasoning and impact.
Lutz Müller,Yasumoto Kikuchi,Gregory S. Probst,Leonard Schechtman,Hiroyasu Shimada,Toshio Sofuni,David Tweats +6 more
TL;DR: The authors are fully aware of the fact that some of the recommendations given in these ICH guidances are transient in nature and that the dynamic qualities and ongoing evolution of genetic toxicology makes necessary a continuous maintenance process that would serve to update the guidance as necessary.
Journal ArticleDOI
Evaluation of the mouse lymphoma tk assay (microwell method) as an alternative to the in vitro chromosomal aberration test
Masamitsu Honma,Makoto Hayashi,Hiroyasu Shimada,Noriho Tanaka,Shinobu Wakuri,Takumi Awogi,Koichi I. Yamamoto,Noriko Ushio Kodani,Yoshisuke Nishi,Masahiro Nakadate,Toshio Sofuni +10 more
TL;DR: It is concluded that the MLA was not as sensitive as the CA because some MLA-negative chemicals evoked positive responses in the CA only after long continuous treatment, and improvement of the MLA protocol, including alteration of the duration of the treatment, might render the MLA as sensitive.
Journal ArticleDOI
The L5178Y/tk+/− mouse lymphoma specific gene and chromosomal mutation assay: A phase III report of the U.S. environmental protection agency Gene-Tox program
TL;DR: The L5178Y/tk+/−-3.7.2C mouse lymphoma assay (MLA) as mentioned in this paper detects mutations affecting the heterozygous thymidine kinase (tk) locus is capable of responding to chemicals acting as clastogens as well as point mutagens.
Journal ArticleDOI
Chromosome 11 aberrations in small colony L5178Y TKPsu−/− mutants early in their clonal history
TL;DR: A cytogenetic technique that allows observation of chromosome rearrangements associated with TK-/- mutagenesis of the L5178Y/TK+/-3.7.2C cell line early in mutant clonal history shows that the major proportion of small-colony (sigma) mutants studied have chromosome 11 rearrangement while large-colonies (lambda) mutants do not have detectable chromosome rearranged.
References
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Journal ArticleDOI
Localization of heterochromatin in human chromosomes
Frances E. Arrighi,T. C. Hsu +1 more
TL;DR: Heterochromatic regions in chromosomes of man, mainly at the centromeric areas, can be demonstrated with consistency using a special staining procedure.
Journal ArticleDOI
Validation and characterization of the L5178Y/TK+/- mouse lymphoma mutagen assay system.
TL;DR: Characterization of the TK-/- mutants suggests that two mutagenic mechanisms contribute to their final yield, and is consistent with the induction of slow-growing specific locus mutants by a chromosomal mechanism and their subsequent dilution during this long expression time.
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A system of nomenclature for band patterns of mouse chromosomes.
Muriel N. Nesbitt,Uta Francke +1 more
TL;DR: Mouse chromosomes banded by quinacrine mustard staining, by the ASG technique, or by Giemsa staining following trypsinization or chymotrypsinizing are described in detail.
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Probable assignment of the Duffy blood group locus to chromosome 1 in man.
TL;DR: Evidence is presented that the Duffy blood group locus Fy, already believed to be linked to a congenital cataract locus4 Cae, is close to this uncoiler element Un, and that all three are probably on chromosome no. 1.
Journal ArticleDOI
Cytogenetic analysis of the L5178Y/TK+/− → TK−/− mouse lymphoma mutagenesis assay system
TL;DR: It seems likely that λ and σ mutants result from 2 different mutational mechanisms that may be distinguished on the basis of mutant colony morphology, possibly related to the type of chromosomal damage sustained.