Journal ArticleDOI
Detection of Schistosoma mansoni in Biomphalaria using nested PCR.
TLDR
A nested polymerase chain reaction (PCR) protocol was developed for detecting the presence of Schistosoma mansoni sporocysts in intermediate host snails of the genus Biomphalaria and has utility in determining if snails in endemic areas bear prepatent or inactive infections and in assessing the degree of compatibility between local snail and schistosome populations.Abstract:
A nested polymerase chain reaction (PCR) protocol was developed for detecting the presence of Schistosoma mansoni sporocysts in intermediate host snails of the genus Biomphalaria. To accomplish this, rDNA genes encoding the 18S rRNA of S. mansoni and Biomphalaria alexandrina from Egypt were sequenced, as were 18S-encoding genes of the 13-16-R1 and Salvador strains of Biomphalaria glabrata. Based on a comparison of host and parasite sequences, a nested set of PCR primers was designed to allow specific amplification of portions of S. mansoni 18S rDNA. These primers allowed detection of as little as 10 fg of S. mansoni DNA diluted in 100 ng of snail DNA and did not allow amplification of snail 18S sequences. Using nested PCR, the presence of a single S. mansoni sporocyst within an adult snail could be detected at 1 day postexposure. In DNA samples extracted from each of 74 snails of the M-line strain of B. glabrata exposed to from 1 to 10 S. mansoni miracidia for intervals ranging from 1 to 44 days, use of the outside primer pair alone detected the parasite's presence in 51% of the snails, whereas the sequential use of outside and nested primer pairs detected parasites in 92% of the snails. This approach has utility in determining if snails in endemic areas bear prepatent or inactive infections and in assessing the degree of compatibility between local snail and schistosome populations. It will also facilitate studies of resistance of snails to infection.read more
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Dissertation
Diseño, desarrollo y aplicación de la tecnología LAMP para el diagnóstico de la esquistosomosis: del laboratorio al campo
TL;DR: In this paper, a tecnica LAMP e uma amplificacao de ADN for the detection of Schistosomamansoni and caracois infected with S.mansoni is presented.
Assessment of Infected Biomphalaria alexandrina Snails by Detecting Schistosoma mansoni Antigen and Specific Gene.
TL;DR: In spite of the superiority and the higher specificity of the immunodetection for larg scale detection of prepatency of B. alexandrina snails infected with S mansoni, the nes ted PCR assay revealed much higher sens itivity which enables 100% detection o f S .
Dissertation
Desenvolvimento de métodos moleculares baseados em PCR para a detecção de Schistosoma mansoni
Journal Article
Discrimination between susceptible and non-susceptible Biomphalaria alexandrina snails -intermediate hosts of Schistosoma mansoni in Western Saudi Arabia -using random amplified polymorphic DNA analysis
TL;DR: It is shown that the RAPD markers method has proved useful in discrimination between susceptible and non-susceptible snails of Biomphalaria alexandrina.
Posted ContentDOI
Using environmental DNA for the detection of Schistosoma mansoni: toward improved environmental surveillance of schistosomiasis
Mita E. Sengupta,Micaela Hellström,H. C. Kariuki,Annette Olsen,Philip Francis Thomsen,Philip Francis Thomsen,Helena Mejer,Eske Willerslev,Eske Willerslev,Eske Willerslev,Mariam T. Mwanje,Henry Madsen,Thomas K. Kristensen,Anna-Sofie Stensgaard,Birgitte J. Vennervald +14 more
TL;DR: It is concluded that eDNA provides a promising new tool to significantly improve the environmental surveillance of S. mansoni and could become an essential future component of the schistosomiasis control tool box needed to achieve the goal of elimination.