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Different media requirements for micropropagation of apricot cultivars

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TLDR
In this article, factors affecting in vitro propagation of several apricot cultivars were studied and the effect of nutrient media and BA concentration were strongly genotype dependent, with best results obtained between 1.78 and 3.11 μM.
Abstract
Factors affecting in vitro propagation of several apricot cultivars were studied. The effect of nutrient media and BA concentration were strongly genotype dependent. Generally, best results were achieved with Quoirin and Lepoivre (1977) and a modification of WPM macronutrients (Lloyd and McCown, 1981). Optimum BA concentration was different for each cultivar but best results were obtained between 1.78 and 3.11 μM. Apricot shoots rooted well with different concentrations of IBA but most shoots showed symptoms of apical necrosis that could be overcome by dipping the shoot tips in solutions of 22.2 or 44.4 μM of BA prior to transfer to rooting medium.

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Citations
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Journal ArticleDOI

Solving the problem of shoot-tip necrosis in Harpagophytum procumbens by changing the cytokinin types, calcium and boron concentrations in the medium

TL;DR: Optimum shoot multiplication was achieved by omitting auxin and using the cytokinin meta -Topolin riboside, and Rooted plantlets produced, using the optimized protocol, were acclimatized successfully by direct transfer to the greenhouse in a 1:1 ratio of sand:soil mixture.
Journal ArticleDOI

Effect of meta-Topolin on micropropagation and adventitious shoot regeneration in Prunus rootstocks

TL;DR: The effect of meta-Topolin (mT), an aromatic natural cytokinin, on micropropagation and adventitious shoot regeneration was evaluated on Prunus rootstocks, Torinel (Prunus domestica L.) and Ferdor (PRunus insititia ǫ× domestica), in vitro shoots were grown for three subcultures on a multiplication medium containing 2.1, 4.2 or 6.3μm of mT or 2.6-benzyladenine (BA) as mentioned in this paper.
Journal ArticleDOI

Ethylene inhibitors and low kanamycin concentrations improve adventitious regeneration from apricot leaves.

TL;DR: With this improved procedure, regeneration from apricot leaves has been increased more than 200% as compared with rates reported previously.
Journal ArticleDOI

Factors contributing to in vitro shoot-tip necrosis and their physiological interactions

TL;DR: This review article summarizes the literature published on the causes of in vitro STN in plants such as medium type, plant growth regulators, calcium, boron, medium additives, the culture environment, their interaction and physiological effects.
Journal ArticleDOI

Adventitious Shoot Regeneration and Rooting of Prunus serotina In Vitro Cultures

TL;DR: A complete regeneration protocol was developed for Prunus serotina Ehrh.
References
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Journal ArticleDOI

A revised medium for rapid growth and bio assays with tobacco tissue cultures

TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Book

Plant Propagation: Principles and Practices

TL;DR: This book discusses Propagation Methods and Rootstocks for the Important Fruit and Nut Species, and theoretical Aspects of Grafting and Budding, and Principles of Tissue Culture for Micropropagation.
Book

Plant Propagation by Tissue Culture

TL;DR: The Anatomy and Morphology of Tissue Cultured Plants M.V. Moshkov, G. V. Novikova, M. Stasolla, E. Zazimalova and E.F. George reveal the secrets of successful tissue culture and the challenges faced in implementing and sustaining such a system.
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