Direct Shoot Regeneration from Vaccinium pahalae (Ohelo) and V. myrtillus (Bilberry) Leaf Explants
Rida A. Shibli,M. A. L. Smith +1 more
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Regenerants were successfully micropropagated, although callus formation caused by zeatin and high 2iP levels interfered with shoot proliferation, and cells from callus and suspension cultures did not exhibit regeneration in any of the media that supported organogenesis from leaves.Abstract:
Ohelo (V. pahalae Skottsb.) and bilberry (V. myrtillus L.) shoots were regenerated via direct organogenesis from whole leaves and leaf sections and also from hypocotyl explants of bilberry. Explants preincubated for 1 to 2 weeks in darkness yielded 75% regeneration frequencies and the highest number of regenerating shoots/explant on TDZ-supplemented media (0.9 to 2.7 μM). When 2iP or zeatin were substituted as the cytokinin source, frequencies of regeneration and shoot productivity were significantly lower. Explants held under constant illumination (no dark pretreatment) had significantly lower regeneration frequencies in all tested cytokinin-supplemented media. 2,4-D stimulated callus formation, but did not support regeneration from vegetative explants. Cells from callus and suspension cultures did not exhibit regeneration in any of the media that supported organogenesis from leaves. Regenerants were successfully micropropagated, although callus formation caused by zeatin and high 2iP levels interfered with shoot proliferation. Zeatin induced hyperhydricity in shoots from both species, but more severely in ohelo. Ex vitro rooting after treatment with 4.9 μM IBA or 5.4 μM NAA was 95 % and 60% successful for bilberry and ohelo, respectively, and plants were readily acclimatized after an interval in a fog chamber. Bilberry microshoots also rooted in vitro in the absence of growth regulator treatment. Chemical names used: 1H-indole-3-butanoic acid (IBA); N-(3-methyl-2-butenyl)-1-H-purine-6-amine (2iP); 6-furfurylaminopurine (kinetin); 1-naphthaleneacetic acid (NAA); thidiazuron=1-phenyl-3-(1,2,3-thiadiazio-5-yl)urea (TDZ); 2,4-dichlorophenoxyacetic acid (2,4-D); 6-(4-hydroxy-3-methylbut-2-enylamino) purine (zeatin).read more
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Journal ArticleDOI
In vitro shoot regeneration from cotyledonary node explants of a multipurpose leguminous tree, pterocarpus marsupium roxb.
Suresh Chand,Ajay Kumar Singh +1 more
TL;DR: A protocol has been developed for in vitro plant regeneration from cotyledonary nodes of Pterocarpus marsupium Roxb and about 52% of plantlets were successfully acclimatized and established in pots.
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Improved shoot organogenesis from leaf explants of highbush blueberry
X. Cao,F.A. Hammerschlag +1 more
TL;DR: Regeneration of 'Duke', 'Jersey', and 'Sierra' on zeatin riboside was significantly better under a PPF of 55 ± 5 μmol·m -2 ·s -1 than under 18 ± 5μmol ·m -1, but the higher PPF inhibited regeneration of ' Duke' on 5 μM TDZ.
Journal ArticleDOI
A Two-step Procedure for Adventitious Shoot Regeneration from in vitro-derived Lingonberry Leaves: Shoot Induction with TDZ and Shoot Elongation Using Zeatin
TL;DR: The effects of TDZ (0, 0.1, 1, 5 and 10 μM) andExplant orientation on adventitious shoot regeneration of 'Eratedank' lingonberry were studied and bud and shoot regeneration was greatly influenced by explant orientation.
Journal ArticleDOI
Strawberry sepal : Another explant for thidiazuron-induced adventitious shoot regeneration
TL;DR: An efficient system to regenerate shoots on excised sepals (calyx) of greenhouse-grown ‘Bounty’ strawberry (Fragaria x ananassa Duch.) was developed in vitro and sepals proved more effective than the leaf discs and petiole segments for regenerating shoots.
Journal ArticleDOI
Effect of N6-isopentenyladenine concentration on growth initiation in vitro and rooting of bilberry and lingonberry microshoots
TL;DR: Spring was a considerably better time than autumn for the initiation of new growth, for both species and the results of the rooting test showed that the KIBA-treatment before planting on peat increases rooting efficiency in both bilberry and lingonberry.
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