Journal ArticleDOI
Distribution of similar self-incompatibility (S) haplotypes in different genera, Raphanus and Brassica
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TLDR
The nucleotide sequences of ten SP11 and nine SRK alleles in Raphanus sativus and Brassica suggest that these S haplotype pairs originated from the same ancestral S haplotypes.Abstract:
The nucleotide sequences of ten SP11 and nine SRK alleles in Raphanus sativus were determined, and deduced amino acid sequences were compared with those of Brassica SP11 and SRK. The amino acid sequence identity of class-I SP11s in R. sativus was about 30% on average, the highest being 52.2%, while that of the S domain of class-I SRK was 77.0% on average and ranged from 70.8% to 83.9%. These values were comparable to those of SP11 and SRK in Brassica oleracea and B. rapa. SP11 of R. sativus S-21 was found to be highly similar to SP11 of B. rapa S-9 (89.5% amino acid identity), and SRK of R. sativus S-21 was similar to SRK of B. rapa S-9 (91.0%). SP11 and SRK of R. sativus S-19 were also similar to SP11 and SRK of B. oleracea S-20, respectively. These similarities of both SP11 and SRK alleles between R. sativus and Brassica suggest that these S haplotype pairs originated from the same ancestral S haplotypes.read more
Citations
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Journal ArticleDOI
Self-incompatibility in Brassicaceae crops: lessons for interspecific incompatibility
TL;DR: A model in which a universal pollen-inhibition pathway is shared by these two incompatibility systems is proposed, which is a mechanism that allows stigmas to recognize and discriminate against “self” pollen, thus preventing self-fertilization and inbreeding.
Journal ArticleDOI
Evolution under strong balancing selection: how many codons determine specificity at the female self-incompatibility gene SRK in Brassicaceae?
Vincent Castric,Xavier Vekemans +1 more
TL;DR: The signature of balancing selection can be identified reliably at the codon level even in cases of very high sequence divergence, provided that a sufficiently large set of sequences are analyzed.
Journal ArticleDOI
Sporophytic self-incompatibility genes and mating system variation in Arabis alpina
TL;DR: The results strongly suggest that, as with other species in the Brassicaceae, A. alpina has a sporophytic SI system but shows variation in the strength of SI within and between populations.
Journal ArticleDOI
Diversification and Alteration of Recognition Specificity of the Pollen Ligand SP11/SCR in Self-Incompatibility of Brassica and Raphanus
TL;DR: Results suggest that Regions III and Region V of SP11 are important for determining the recognition specificity, but not the sole determinant.
Journal ArticleDOI
Self-(In)compatibility Systems: Target Traits for Crop-Production, Plant Breeding, and Biotechnology.
TL;DR: Scientific progress as well as patented applications of SI are reviewed, and future prospects are highlighted including further elucidation of SI systems, deepening the understanding of SI-environment relationships, and new perspectives on plant self/non-self recognition.
References
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Journal ArticleDOI
The male determinant of self-incompatibility in Brassica.
TL;DR: Loss- of-function and gain-of-function studies prove that the SCR gene product is necessary and sufficient for determining pollen self-incompatibility specificity, possibly by acting as a ligand for the stigmatic receptor.
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Molecular cloning of a putative receptor protein kinase gene encoded at the self-incompatibility locus of Brassica oleracea
TL;DR: The S receptor kinase (SRK) gene is described, a previously uncharacterized gene that resides at the S locus that exhibits striking homology to the secreted product of the S-locus glycoprotein (SLG) gene.
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The S receptor kinase determines self-incompatibility in Brassica stigma.
TL;DR: It is shown that by transforming self-incompatible plants of Brassica rapa with an SRK28 and an SLG 28 transgene separately, that expression of SRK 28 alone, but not SLG28 alone, conferred the ability to reject self (S28)-pollen on the transgenic plants, and that the ability ofSRK28 to reject S28 pollen was enhanced bySLG 28.
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Self-incompatibility systems in angiosperms
TL;DR: In this article, the authors propose a method to solve the problem of the problem: this article...,.. ].. ).. ]... )...
Journal ArticleDOI
Direct ligand–receptor complex interaction controls Brassica self-incompatibility
Seiji Takayama,Hiroko Shimosato,Hiroshi Shiba,Miyuki Funato,Fang-Sik Che,Masao Watanabe,Megumi Iwano,Akira Isogai +7 more
TL;DR: It is shown that a single form of SP11 of the S8 haplotype (S8-SP11) stabilized with four intramolecular disulphide bonds specifically binds the stigma membrane of theS8 haplotypes to induce autophosphorylation of SRK8, and thatSRK8 and SLG8 together form a high-affinity receptor complex for S8- SP11 on the stigma membranes.