Journal ArticleDOI
Dynamic Speckle-Interferometry of Microscopic Processes in Thin Biological Objects
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In this paper, a model that allows for random time variations in the phase difference Δφ of pairs of waves transmitted through the object is used in the theoretical part of this study.Abstract:
Speckle dynamics in a thin biological object illuminated through a diffusor box is studied both theoretically and experimentally. A model that allows for random time variations in the phase difference Δφ of pairs of waves transmitted through the object is used in the theoretical part of this study. Formulas for the time-averaged intensity of the radiation at a certain point of observation and a temporal autocorrelation function of the radiation intensity are deduced. The relationship between the characteristics of the random variable Δφ and the parameters of the speckle dynamics is discussed. The case of occurrence of a quasiperiodic variation in the radiation intensity is separately considered. The theoretical results are used for interpretation of the data obtained in the experiments on studying the metabolic activity of cells cultivated on a glass substrate.read more
Citations
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Journal ArticleDOI
Speckle metrology of dynamic macro- and microprocesses in deformable media
TL;DR: In this article, a theory of the speckle dynamics arises in the image plane of the object as a result of deterministic and random displacements of point scattering centers are considered.
Proceedings ArticleDOI
Applicaton of speckle dynamics for studies of cell metabolism
Abstract: Previously, a speckle interferometry technique and a device that allows quantitative evaluation of the metabolic activity of cultured cells were theoretically grounded and successfully tested. A speckle time-averaging technique was proposed to separate and study the processes occurring in cells at various velocities. The objective of the present research was comparing the parameters of speckle dynamics used to evaluate cell metabolism and averaged in areas of various size. Areas inside the speckle image of a cell as well as areas of the image plane with various numbers of cells were averaging areas. The target of the research was cells from L-41 culture placed onto a special optical tray with a nutrient solution immediately after defrosting. Time-average value T of digital radiation intensity in the TV camera pixels I (1) and the correlation coefficient of two digital images η (2) were used as speckle field change parameters. The digital images corresponded to a single frame area at two time moments. It is shown that in the area containing hundreds of cells dependence η(t) levels off, which indicate stationarity of random value I~ . Features of η(t) dependences obtained by averaging over large and small cell numbers image are discussed. Using the data obtained, we formulated recommendations on selecting area sizes for averaging physical values to study various processes occurring in cells.
Book ChapterDOI
Dynamic Speckle Interferometry of Thin Biological Objects: Theory, Experiments, and Practical Perspectives
TL;DR: In this paper, a model dealing with interference of multiple waves with random phases was used to detect the phase dynamics of the waves sounding a thin biological object and the dynamics of speckles in the object image plane.
Journal ArticleDOI
Dynamic Speckle Interferometry of Microscopic and Macroscopic Processes in Deformable Media
TL;DR: In this article, the theory of the dynamic variant of optical speckle interferometry and its application to study in real-time macroscopic and microscopic processes in deformable media is briefly considered.
Proceedings ArticleDOI
Dynamic speckle interferometry of high-cycle material fatigue: Theory and some experiments
TL;DR: In this paper, a theoretical analysis of speckle dynamics in the image plane of a thin transparent object is presented, where it is suggested that speckles develop in simultaneous periodic motion of the sample, micro-and macro-variations of its refraction index and its translational motion.
References
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Journal ArticleDOI
Interferometric displacement measurement on scattering surfaces utilizing speckle effect
TL;DR: In this article, the distribution of intensity in the resultant pattern depends on the relative phases of the component patterns, and by measuring the correlation between the resultant patterns at two different times a change of relative phase is detected.
Journal ArticleDOI
Speckle Displacement and Decorrelation in the Diffraction and Image Fields for Small Object Deformation
TL;DR: In this paper, the cross-correlation functions of speckle intensities before and after deformation were investigated by calculating the crosscorrelation function of the intensity distribution of laser-speckles.