Journal ArticleDOI
Effect of polybrene on the N-terminal sequencing of peptides bound to polyvinylidene difluoride membranes
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This article is published in Analytical Biochemistry.The article was published on 1996-05-15. It has received 4 citations till now.read more
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Journal ArticleDOI
Binding and detection of glycosaminoglycans immobilized on membranes treated with cationic detergents.
TL;DR: The detection sensitivity was equal for different types of GAGs but there was a slight negative interference from fibrinogen in blood plasma, urine, or cerebrospinal fluid.
Journal ArticleDOI
The removal of pyroglutamic acid from monoclonal antibodies without denaturation of the protein chains
TL;DR: A series of optimization experiments using elevated temperatures and detergents to denature and stabilize the heavy chains of immunoglobulins such that the pyroglutamate at the amino terminal was accessible to enzymatic removal using the thermostable protease isolated from Pyrococcus furiosus.
Journal ArticleDOI
Fluorescence detection of amino acids in the postcleavage conversions for manual sequencing of a peptide.
TL;DR: The suitability of the method was demonstrated by the sensitive manual sequencing of insulin chain B composed of 30 amino acids, which was approximately 10 times higher than ultraviolet absorbance detection of phenylthiohydantoin products in the conventional Edman degradation.
Journal ArticleDOI
Use of ethanolamine for sample stacking in capillary electrophoresis.
TL;DR: Ethanolamine‐CZE allowed the collection of sufficient amounts of pure peptidic material to perform amino acid sequence analysis and was coupled to narrow‐bore reversed‐phase high‐performance liquid chromatography.