Journal ArticleDOI
Electrochemical potential of free and immobilized Cratylia mollis seed lectin.
S.R Souza,Rosa F. Dutra,Maria Tereza dos Santos Correia,M.M.A Pessoa,J.L Lima-Filho,Luana Cassandra Breitenbach Barroso Coelho +5 more
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TLDR
Electrochemical potentials for free or immobilized Cratylia mollis seed lectin (Cra) were obtained through potentiostatic techniques and a linear behavior in relation to the increase in glucose concentration was described.About:
This article is published in Bioresource Technology.The article was published on 2003-07-01. It has received 9 citations till now. The article focuses on the topics: Saturated calomel electrode & Electrochemical potential.read more
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Book ChapterDOI
Protein Purification by Affinity Chromatography
Luana Cassandra Breitenbach Barroso Coelho,Andréa F. S. Santos,Thiago Henrique Napoleão,M.T.S. Correia,Patrícia Maria Guedes Paiva +4 more
TL;DR: In immunoaffinity chromatography an antibody or an antigen is immobilized on a support so as to purify the protein against which the antibody was developed to achieve optimal protein purification with maximum recovery.
Journal ArticleDOI
Ferromagnetic Levan Composite: An Affinity Matrix to Purify Lectin
Renata Angeli,Renata Angeli,Nathalia V. N. da Paz,J. C. Maciel,Flavia F. B. Araújo,Patrícia Maria Guedes Paiva,Glícia Maria Torres Calazans,Ana Paula Valente,Fabio C. L. Almeida,Luana Cassandra Breitenbach Barroso Coelho,Luiz Felipe Pereira Borba Carvalho,Luiz Felipe Pereira Borba Carvalho,Maria da Paz Carvalho da Silva,Maria da Paz Carvalho da Silva,Maria Tereza dos Santos Correia +14 more
TL;DR: Cramoll 1 was purified by this affinity binding procedure in two steps instead of a previous three-step protocol with ammonium sulfate fractionation, affinity chromatography on Sephadex G-75, and ion exchange chromatography through a CM-cellulose column.
Journal ArticleDOI
Quantum dot–Cramoll lectin as novel conjugates to glycobiology
Cássia R.A. Cunha,Camila G. Andrade,Maria I.A. Pereira,Paulo E. Cabral Filho,Luiz B. Carvalho,Luana Cassandra Breitenbach Barroso Coelho,Beate S. Santos,Adriana Fontes,Maria Tereza dos Santos Correia +8 more
TL;DR: The optical properties of quantum dots (QDs) make them useful tools for biology, especially when combined with biomolecules such as lectins, and QDs conjugated to Cramoll at pH7.0 showed the best labeling efficiency in the fluorescence microscopy analysis.
Journal ArticleDOI
The Cratylia mollis seed lectin induces membrane permeability transition in isolated rat liver mitochondria and a cyclosporine a-insensitive permeability transition in Trypanosoma cruzi mitochondria.
Mariana P. Fernandes,Ana Catarina Rezende Leite,Flavia F. B. Araújo,Sara T.O. Saad,Mariana Ozello Baratti,M.T.S. Correia,Luana Cassandra Breitenbach Barroso Coelho,Fernanda Ramos Gadelha,Anibal E. Vercesi +8 more
TL;DR: Results indicate that Cramoll 1, 4 leads to inner mitochondrial membrane permeabilization through Ca2+ dependent mechanisms in both mitochondria, indicating a CsA‐insensitive MPT in T. cruzi mitochondria.
Journal ArticleDOI
Electrochemical potential of Microgramma vaccinifolia rhizome lectin
Giselly Maria de Sá Santana,Lidiane Pereira de Albuquerque,Thiago Henrique Napoleão,Sandra Rodrigues de Souza,Luana Cassandra Breitenbach Barroso Coelho,Patrícia Maria Guedes Paiva +5 more
TL;DR: The study reveals that the stimulatory effect of Ca�⁺ and Mg²⁺ on hemagglutinating activity may be linked to conformational change and/or alterations in surface charge distribution of MvRL.
References
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Journal ArticleDOI
Materials and techniques for electrochemical biosensor design and construction
S.F Zhang,G Wright,Ying Yang +2 more
TL;DR: This review aims to highlight recent developments in materials and techniques for electrochemical biosensor design and construction, raising hopes that the problems related to interactions between foreign surfaces and biological fluids and tissues may soon be solved.
Journal ArticleDOI
Purification of a Glucose/Mannose Specific Lectin, Isoform 1, from Seeds of Cratylia mollis Mart. (Camaratu Bean)
TL;DR: A quantitatively main molecular form ofCratylia mollis lectin, isoform 1 (iso 1) was purified by affinity chromatography on Sephadex G-75, followed by ion exchange chromatographyon CM-cellulose, and another lectin form was identified in the latter step.
Journal ArticleDOI
Revisiting proteus: do minor changes in lectin structure matter in biological activity? Lessons from and potential biotechnological uses of the Diocleinae subtribe lectins
TL;DR: The present review of the biological actions of Diocleinae lectins includes several in vitro and in vivo immunological findings, as well as their effects on insect growth and reproduction.
Journal ArticleDOI
A micro-planar amperometric glucose sensor unsusceptible to interference species
TL;DR: In this article, the authors developed an amperometric glucose sensor in which a hydrogen peroxide detecting platinum electrode is coated with γ -APTES, acetylcellulose, Nafion®, glucose oxidase, and polydimethylsiloxane to prevent interference species.
Journal ArticleDOI
Binding evaluation of Isoform 1 from Cratylia mollis lectin to human mammary tissues.
Eduardo Isidoro Carneiro Beltrão,Maria Tereza dos Santos Correia,José Figueredo-Silva,Luana Cassandra Breitenbach Barroso Coelho +3 more
TL;DR: Neoplastic tissues, marked by Cra Iso 1, showed a higher intensity of staining than normal ones, in comparison with Canavalia ensiformis lectin, Concanavalin A, conjugated to peroxidase (Con A-Per).
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