Open AccessJournal Article
Fasciola hepatica and Dicrocoelium dendriticum: isoenzyme patterns of malate dehydrogenase and malic enzyme.
TLDR
Only in F. hepatica were differences observed between the enzyme profiles of specimens obtained from the different host species, and the densitometric scans in samples from specimens from differenthost species.Abstract:
The MDH isoenzymes and ME isoenzymes (EC.1.1.1.39) in two helminth parasites, F. hepatica and D. dendriticum, obtained from two host species (Capra hircus and Ovis aries), were studied by electrophoresis on polyacrylamide gels. F. hepatica MDH showed three isoenzymatic bands; D. dendriticum MDH, only two. No difference was noted between the enzyme profiles, or the densitometric scans in samples from specimens from different host species. F. hepatica ME presented three bands, that of D. dendriticum, only two. Only in F. hepatica were differences observed between the enzyme profiles of specimens obtained from the different host species.read more
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Journal ArticleDOI
Molecular variation in fasciolids and Paragonimus.
TL;DR: Molecular variation, both intra- and inter-specific, in fasciolids and Paragonimus has been detected in isozymes and other proteins, antigens and nucleic acid sequences, for studies on population genetics, taxonomy, phylogeny, immunodiagnosis, ontogeny and host-induced variation.
Journal ArticleDOI
Preliminary study on genetic variability of Dicrocoelium dendriticum determined by random amplified polymorphic DNA
Hilda Sandoval,M. Yolanda Manga-González,Raquel Campo,Pedro Escobar García,José M. Castro,M. Pérez de la Vega +5 more
TL;DR: Genetic variability seems to be high in this parasite and genetic similarity within a population (worms infecting a single animal) is similar to the average similarity between worms from different sheep.
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Characterization of Dicrocoelium dendriticum haplotypes from sheep and cattle in Iran based on the internal transcribed spacer 2 (ITS-2) and NADH dehydrogenase gene (nad1).
TL;DR: A phylogenetic analysis of the sequence data revealed that host associations and geographic location are not likely useful markers for D. dendriticum haplotype classification and sequencing results obtained from the nad1 gene as a mitochondrial marker for the first time in this study would provide a valuable tool to analyse further molecular details of the species worldwide.
Journal ArticleDOI
Characterization of adult Dicrocoelium dendriticum by isoelectric focusing
TL;DR: Water soluble extracts of 3131 adult specimens of Dicrocoelium dendriticum from cattle, sheep and goats, mainly from León province, were analysed by isoelectric focusing in thin-layer polyacrylamide gels, finding little phenotypic variation was observed between worms recovered from a single host.