Patent
Fluidic assembly for an ultra-high-speed chromosome flow sorter
TLDR
In this article, a fluidic assembly for an ultra-high-speed chromosome flow sorter using a fluid drive system, a nozzle with an orifice having a small ratio of length to diameter, and a mechanism for vibrating the nozzle along its axis at high frequencies is presented.Abstract:
A fluidic assembly for an ultra-high-speed chromosome flow sorter using a fluid drive system, a nozzle with an orifice having a small ratio of length to diameter, and mechanism for vibrating the nozzle along its axis at high frequencies. The orifice is provided with a sharp edge at its inlet, and a conical section at its outlet for a transition from a short cylindrical aperture of small length to diameter ratio to free space. Sample and sheath fluids in separate low pressure reservoirs are transferred into separate high pressure buffer reservoirs through a valve arrangement which first permit the fluids to be loaded into the buffer reservoirs under low pressure. Once loaded, the buffer reservoirs are subjected to high pressure and valves are operated to permit the buffer reservoirs to be emptied through the nozzle under high pressure. A sensor and decision logic is positioned at the exit of the nozzle, and a charging pulse is applied to the jet when a particle reaches a position further downstream where the droplets are formed. In order to adjust the timing of charge pulses, the distance between the sensing station at the outlet of the nozzle and the droplet breakoff point is determined by stroboscopic illumination of the droplet breakoff region using a laser and a revolving lucite cylinder, and a beam on/off modulator. The breakoff point in the region thus illuminated may then be viewed, using a television monitor.read more
Citations
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Patent
Method and apparatus for sorting particles
TL;DR: In this article, a method and apparatus for sorting particles moving through a closed channel system of capillary size comprises a bubble valve for selectively generating a pressure pulse to separate a particle having a predetermined characteristic from a stream of particles.
Patent
Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm
Gary Durack,Jeffrey D. Wallace,Gary P. Vandre,Lon A. Westfall,Jeremy T. Hatcher,Niraj V. Nayak +5 more
TL;DR: In this article, a multi-channel system for classifying particles in a mixture of particles according to one or more characteristics including a common source of electromagnetic radiation for producing a beam of radiation and a beam splitter for producing multiple beams of radiation for each interrogation location associated with each flow channel of the system.
Patent
Method and apparatus for flow cytometry
TL;DR: A flow cytometer (20) capable of utilizing an imaging system including a sensor (88) to determine various properties of the flow cytometers (20). Importantly, the imaging allows for determination of a drop delay time (154), other characteristics that can be determined include at least the width of the stream, pressure of the streams, effect of charged droplets on other droplets (44), trajectory of a stream, resonant frequency, wavelengths, change in position of droplet break-off point (150), and others as discussed by the authors.
Patent
Multiple sort flow cytometer
TL;DR: A flow cytometer utilizes multiple lasers for excitation and respective fluorescence of identified dyes bonded to specific cells or events to identify and verify multiple events to be sorted from a sheath flow and droplet stream.
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Flow cytometry nozzle for high efficiency cell sorting
TL;DR: A sorting nozzle with two tapered zones (8, 9) with elliptical cross sections is able to orient and sort a large fraction of asymmetrical or 'flattened' cells as discussed by the authors.
References
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Patent
Measurement of angular dependence of scattered light in a flowing stream
TL;DR: In this article, a particle analyzing method and apparatus are provided for measuring the intensity of light scattered from a particle as a function of angle, where a beam of collimated monochromatic radiation is directed onto the flow path and detector means are provided having a radiation acceptance geometry whose projection intercepts the beam along the scatter line.
Patent
Single drop separator
TL;DR: In this article, a fluid stream is formed in air and an electrical charge or radiant energy is applied to the stream at a particular location in order to disturb the stream and create a single drop.
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Spraying atomized particles
TL;DR: In this paper, the authors present a process for spraying atomized particles, which comprises producing a stream of gas atomised particles and directing a secondary stream of gases against the stream of atomized gas atoms and imparting an oscillation substantially.
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Apparatus for the formation of liquid droplets
TL;DR: In this article, an apparatus and method for the production of particles wherein liquid droplets, to be formed into particles, are produced by the ejection of liquid out of the orifices of a spinnerette which is vibrated.