Journal ArticleDOI
Glycerolpropylsilane bonded phases in the steric exclusion chromatography of biological macromolecules.
Fred E. Regnier,R. Noel +1 more
TLDR
It has been demonstrated that carbohydrate bonded supports may be used in the chromatography of proteins, nucleic acids, and polysaccharides.Abstract:
Glycerolpropylsilane bonded phases have been found to control the adsorption and/or denaturation of proteins and nucleic acids on controlled porosity glass supports. The bonded-phase thickness is 18-19A while the amount of glycerol moiety varies from 80 to 150 mumoles/g depending on support pore diameter. It has been demonstrated that carbohydrate bonded supports may be used in the chromatography of proteins, nucleic acids, and polysaccharides.read more
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Journal ArticleDOI
Hydrophilic interaction liquid chromatography (HILIC)—a powerful separation technique
Bogusław Buszewski,Sylwia Noga +1 more
TL;DR: Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to effectively separate small polar compounds on polar stationary phases and their applications for separations of polar compounds in complex matrices.
Journal ArticleDOI
Stationary and mobile phases in hydrophilic interaction chromatography: a review
TL;DR: This review deals with recent advances in the development of HILIC phase separation systems with special attention to the properties of stationary phases.
Journal ArticleDOI
Size-Exclusion Chromatography for the Analysis of Protein Biotherapeutics and their Aggregates
TL;DR: The history and background of SEC for the analysis of proteins and some of the applications of this mode of separation that are of particular importance to protein biopharmaceutical development are reviewed and some considerations in their implementation are highlighted.
Journal ArticleDOI
Separation efficiencies in hydrophilic interaction chromatography.
TL;DR: This review discusses the column efficiency of HILIC materials in relation to solute and stationary phase structures, as well as comparisons between particle-packed and monolithic columns.
Book ChapterDOI
High-performance liquid chromatography of proteins
Fred E. Regnier,Karen M. Gooding +1 more
TL;DR: A review of recent studies on high-performance liquid chromatography (hplc) of proteins in gel permeation, ion exchange, reversed phase, normal phase, and affinity modes is presented in this article.