Glycolate Uptake by Mutant Strains of Escherichia coli K-12
L N Ornston,M. K. Ornston +1 more
TLDR
Mutant strains of Escherichia coli K-12 were shown to be impaired in their ability to assimilate glycolate-2-(14)C, and one strain was deranged in the synthesis of either glyoxylate reductase or malate synthase G.Abstract:
Mutant strains of Escherichia coli K-12 were shown to be impaired in their ability to assimilate glycolate-2-(14)C. One strain (Glc-103) has lost the ability to oxidize glycolate; another strain (Glc-102) was relatively impermeable to the compound. A third strain (Glc-104) had undergone a similar loss in permeability, and, in addition, was deranged in the synthesis of either glyoxylate reductase or malate synthase G.read more
Citations
More filters
Journal ArticleDOI
The uptake of C4-dicarboxylic acids by Escherichia coli.
William W. Kay,H.L. Kornberg +1 more
TL;DR: It is suggested that E. coli effect the uptake of C4-dicarboxylic acids through a common, inducible and highly specific transport system.
Journal ArticleDOI
Characterization of the specific pyruvate transport system in Escherichia coli K-12.
TL;DR: The results indicate that uptake of pyruvate in E. coli is via a specific active transport system that requires an artificial electron donor system, phenazine methosulfate and sodium ascorbate.
Book ChapterDOI
The Genetic Control of Membrane Transport
TL;DR: This chapter discusses the techniques that have been developed to isolate and characterize transport mutants, the criteria for identifying genes that affect transport directly, the linkage relationships of transport mutants in several organisms, the regulation of synthesis of transport systems, and the usefulness of mutants in understanding transport mechanisms.
Journal ArticleDOI
Glycolate metabolism by Pseudomonas sp., strain S227, isolated from a coastal marine sediment
H. M. Edenborn,Carter Litchfield +1 more
TL;DR: The characteristics of Glycolate metabolism observed in Pseudomonas sp.
References
More filters
Journal ArticleDOI
A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter
TL;DR: A modification of the naphthalene-dioxane-PPO liquid scintillator has been described which will allow up to 3.0 ml of an aqueous solution to be counted as mentioned in this paper.
Journal ArticleDOI
The anaplerotic fixation of carbon dioxide by Escherichia coli.
J. M. Ashworth,H.L. Kornberg +1 more
TL;DR: The isolation of a mutant of Escherichia coli K 12 is described, which differs from its parent organism in lacking the ability to grow upon glucose, glycerol or pyruvate unless utilizable intermediates of the tricarboxylic acid cycle are also supplied in the growth media, and it is concluded that the net formation of C4-acids through carboxylation of C3-precursors is necessarily achieved through the agency of phosphopyru
Journal ArticleDOI
Regulation of glyoxylate metabolism in Escherichia coli K-12.
L. N. Ornston,M. K. Ornston +1 more
TL;DR: Evidence indicates that malate synthase G plays an anaplerotic role during growth with glycolate or acetate as the carbon source, and that wild-type organisms convert glyoxylate to acetyl-coenzyme A and oxidize it via the tricarboxylic acid cycle.
Journal ArticleDOI
Physiologie et génétique de l'isocitritase et des malate synthases chez Escherichia coli
E Vanderwinkel,M. de Vlieghere +1 more
TL;DR: The results, together with the properties of the strain constitutive for isocitritase and malate synthase A, point to the occurrence of an ace operon (aceA, aceB) regulated by repression-derepression.