Journal ArticleDOI
Histological processing in autoradiography: loss of radioactivity.
TLDR
An investigation has been carried out on the amount of activity lost from rat and human tissues during fixation and dehydration and various procedures for staining sections before application of photographic emulsion and after developing are discussed.Abstract:
Histological methods suitable for use in autoradiographic technics are described. An investigation has been carried out on the amount of activity lost from rat and human tissues during fixation and dehydration. Losses in the processing fluids varied from 25% to 90% of the initial activity for radioactive phosphorus and 4% to 20% for radioactive iodine in various fixatives.The care necessary in handling sections if distribution of total activity is being studied is emphasized and floating on absolute alcohol is suggested as an alternative to warm mercury. Various procedures for staining sections before application of photographic emulsion and after developing are discussed. Ehrlich's hematoxylin applied regressively has given good results and eosin has been used successfully as a counterstain. Orth's lithium carmine is resistant to photographic developer and also Feulgen's stain counterstained with fast green can be used before covering the slides with photographic emulsion.read more
Citations
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Journal ArticleDOI
The histogenesis of the mouse cerebellum as studied by its tritiated thymidine uptake.
Book ChapterDOI
Die Orthologie und Pathologie des Nukleinsäure- und Eiweißstoffwechsels der Zelle im Autoradiogramm
Journal Article
An autoradiographic study of the penetration of subconjunctivally injected hydrocortisone into the normal and inflamed rabbit eye.
TL;DR: It appears that subconjunctival injections of hydrocorlisone, to produce the maximum effect with the minimum dosage, should be immediately adjacent to the intraocular inflammation under treatment, rather than placing the injection haphazardly or always in the superior temporal quadrant.
Journal ArticleDOI
Stains Compatible with Dipping Radioautography
Jean M. Thurston,David L. Joftes +1 more
TL;DR: Paraffin sections of 13 different kinds of mouse tissues, containing tritiated thymidine, were used to test stains applied either before or after application of the nuclear emulsion by dipping to determine compatibility.
Book ChapterDOI
High-Resolution Autoradiography of H3-Estradiol with Unfixed, Unembedded 1.0µ Freeze-Dried Frozen Sections
Walter E. Stumpf,Lloyd J. Roth +1 more
TL;DR: The autoradiographic localization of labeled compounds imposes severe limitations on the selection of procedures to which the tissue is subjected in the process, and numerous studies of questionable value have been carried out with unsuitable methods.
References
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Journal ArticleDOI
A method for locating radioactive elements in tissues by covering histological sections with a photographic emulsion.
L.F. Belanger,C. P. Leblond +1 more
TL;DR: A method for histological localization of polonium in tissue sections was developed by Lacassagne and Lattes (1924) as mentioned in this paper, where histological sections of a tissue containing a radioactive element were juxtaposed to a photographic film or plate, and an image appeared on the negative.
Journal ArticleDOI
Histological Localization of Newly-formed Desoxyribonucleic Acid.
Journal ArticleDOI
Techniques of autoradiography and the application of the stripping-film method to problems of nuclear metabolism.
S.R. Pelc,Alma Howard +1 more
Journal ArticleDOI
Preparation of Radioautographs of Tissues without Loss of Water-Soluble P32.
Journal ArticleDOI
Preparation of Radioautographs of Thyroid Tumors for Study at High Magnification
TL;DR: One of the methods of studying iodine uptake of thyroid tumors is to make radioautographs of thin sections of the tissue, where the exact location of the radioiodine is difficult to determine, as at higher magnifications the alignment of the two preparations becomes arbitrary.
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A method for locating radioactive elements in tissues by covering histological sections with a photographic emulsion.
L.F. Belanger,C. P. Leblond +1 more