Book ChapterDOI
Human epidermal growth factor
Graham Carpenter,Stanley Cohen +1 more
- pp 83-105
About:
The article was published on 1977-01-01. It has received 2 citations till now. The article focuses on the topics: Growth factor receptor inhibitor.read more
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Reference EntryDOI
Defined Media and Supplements
TL;DR: The description of culture media starts with the physicochemical properties that are required, including control of CO2 and bicarbonate concentration, pH, buffering, oxygen tension, osmolality, temperature, and viscosity, and ends with advice on selecting a suitable medium and batch reservation and testing of serum.
Journal ArticleDOI
Responsiveness of human lung diploid fibroblast ageing in vitro to epidermal growth factor: saturation density and lifespan
Kazuhiko Kaji,Mitsuyoshi Matsuo +1 more
TL;DR: In the first half of their in vitro lifespan the magnitude of the EGF-induced augmentation increased and in the second half, decreased, and the lifespan of human diploid fibroblasts was not extended by continuous exposure to EGF.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Isolation of a Mouse Submaxillary Gland Protein Accelerating Incisor Eruption and Eyelid Opening in the New-born Animal
TL;DR: The isolation of the factor responsible for the earlier development of the incisors and eyelids is reported here, a heat-stable, nondialysable, antigenic protein, whose most distinctive chemical characteristic is the absence of phenylalanine and lysine.
Journal ArticleDOI
"Contact inhibition" of cell division in 3T3 cells.
TL;DR: It is found that the characteristic "contact-inhibited" cell density observed for 3T3 cells is a fortuitous result of growing the cells in a medium that contains 10 per cent calf serum, and that an insulin-like factor in serum is required for cell divison by cultured chick cells.
Journal ArticleDOI
Human epidermal growth factor: isolation and chemical and biological properties
TL;DR: A polypeptide hormone has been isolated from human urine and it was assayed by its ability to compete with 125I-labeled mouse-derived epidermal growth factor in binding to human foreskin fibroblasts.
Journal ArticleDOI
Characterization of the binding of 125-I-labeled epidermal growth factor to human fibroblasts.
TL;DR: It is demonstrated that cells derived from a number of species (human, mouse, rat, and chick) possessed receptors that interacted with this mouse-derived growth factor.