Journal ArticleDOI
Identification of caffeine and theobromine by chromatography in a thin layer of a polyamide
N. N. Lyakina,L. I. Brutko +1 more
TLDR
Chromatography in a thin layer of a polyamide deposited on a support stable towards solvents, i.e., polyethylene terephthalate film, is successfully used in the analytical chemistry of proteins as discussed by the authors.Abstract:
Chromatography in a thin layer of a polyamide deposited on a support stable towards solvents, i.e., polyethylene terephthalate film, is successfully used in the analytical chemistry of proteins. The dinitrophenylation reaction [i], the dansylation reaction [2-4], and the formation of phenylthiohydantoins [5] of peptides and proteins, followed by acid hydrolysis, are widely used for the determination of the N-terminal amino acids. Thin-layer chromatography on a polyamide is used for the separation and identification of the corresponding derivatives of amino acids.read more
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Journal ArticleDOI
Chromatographic identification of dinitrophenylamino acids on polyester film supported polyamide layers
Wang Kung-Tsung,Wang S.I. Iris +1 more
Journal ArticleDOI
A highly sensitive method for amino-acid analysis by a double-isotope-labelling technique using dansyl chloride.
TL;DR: The amino acid composition of a tryptic peptide containing the active site disulphide bridge of the lipoamide dehydrogenase component of the pyruvate dehydrogenases multienzyme complex of Escherichia coli has been shown to be identical to that of the corresponding peptide from the 2-oxoglutarate dehydration complex of the same organism.
Journal ArticleDOI
A dansylation microassay for some amino acids in brain.
Michael H. Joseph,John Halliday +1 more
TL;DR: An improved chromatographic separation of dansyl-taurine is employed and estimates of the contents of these amino acids in rat cortical tissue are reported and compared with those obtained on an autoanalyser, and with those in the literature using autoanalytical and other methods.
Journal ArticleDOI
Quantitative analysis of amino acids and peptides in the femtomolar range
TL;DR: This method was used for the quantitative analysis of the amino acids in a complete enzymic hydrolyzate of the synthetic hexapeptide H-Leu-Trp-Met-Arg-Phe-Ala-OH.