Open AccessJournal Article
Immunolocalization of protein-bound 3-nitrotyrosine in inflammatory myopathies.
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TLDR
The 3NT immunostaining was localized to endothelial cells and their close surroundings in muscle biopsies of DM and PM patients but only in those areas of tissue sections where inflammatory cell infiltrates were present.Abstract:
SUMMARY 3-nitrotyrosine (3NT) is regarded as a “footprint” of nitric oxide generation. The study aimed at documenting the presence and distribution of 3-nitrotyrosine (3NT) in muscle tissue samples from patients with idiopathic inflammatory myopathies (IIM) as well as from those with non-inflammatory myopathies to consider whether polymyositis (PM) and dermatomyositis (DM) could be distinguished based on 3NT immunohistochemistry in muscle biopsy. Cryosections prepared from muscle biopsies of 54 patients with either IIM, i.e. PM and DM, or various non-inflammatory myopathies were immunostained using monoclonal antibody against 3NT. The 3NT immunostaining was localized to endothelial cells and their close surroundings in muscle biopsies of DM and PM patients but only in those areas of tissue sections where inflammatory cell infiltrates were present. No 3NT positivity was found in tissue sections of IIM patients without inflammatory infiltrates in the studied sample as well as in muscle tissue sections of patients with non-inflammatory myopathies. However, the endothelial cells were also positive in cases of confirmed non-inflammatory myopathies with secondary lymphocytic infiltration (myodystrophies, myasthenia gravis). Despite the pathogenetic significance, the 3NT immunohistochemistry is of low diagnostic value for the differential diagnosis of IIM in muscle biopsy.read more
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Oxidative Stress in the Developing Rat Brain due to Production of Reactive Oxygen and Nitrogen Species.
TL;DR: There was a strong correlation between detection of nitrotyrosine and carboxyethyllysine probably caused by lipid peroxidation initiated with RONS, and the internal elastic laminae of blood vessels revealed the blue autofluorescence.
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Proteomic analysis of peroxynitrite-induced protein nitration in isolated beef heart mitochondria.
TL;DR: The high sensitivity of proteins to nitration by peroxynitrite is of potential biological importance, as these enzymes are involved in various pathways associated with energy production in the heart.
Book ChapterDOI
A Method for Analysis of Nitrotyrosine-Containing Proteins by Immunoblotting Coupled with Mass Spectrometry.
Matej Kohutiar,Adam Eckhardt +1 more
TL;DR: In this paper, a protocol for analysis of peroxynitrite-induced protein nitration in isolated mitochondria is described, where 2D electrophoresis and western blotting techniques coupled with mass spectrometry are common methods used in analysis of proteome.
References
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Book
Polymyositis and Dermatomyositis
TL;DR: Early initiation of therapy is essential, since both polymyositis and dermatomyositis respond to immunotherapeutic agents and new immunomodulatory agents currently being tested in controlled trials may prove promising for difficult cases.
Journal ArticleDOI
Physiology of Nitric Oxide in Skeletal Muscle
TL;DR: These studies provide new insights into fundamental aspects of muscle physiology, cell biology, ion channel physiology, calcium homeostasis, signal transduction, and the biochemistry of redox-related systems.
Journal ArticleDOI
119th ENMC international workshop: trial design in adult idiopathic inflammatory myopathies, with the exception of inclusion body myositis, 10-12 October 2003, Naarden, The Netherlands.
Jessica E. Hoogendijk,Anthony A. Amato,Bryan Lecky,Ernest Choy,Ingrid E. Lundberg,Michael R. Rose,Jiri Vencovsky,Marianne de Visser,Richard A. C. Hughes +8 more
TL;DR: This poster presents a poster presented at the 2016 European Congress of Neurology and Neurosurgery entitled “Neurosurgery and Rheumatology: Foundations of Clinical Neurosciences, 2nd Ed.” (23-25 May 2016).