Improvements in epoxy resin embedding methods.
TLDR
Epoxy embedding methods of Glauert and Kushida have been modified so as to yield rapid, reproducible, and convenientembedding methods for electron microscopy.Abstract:
Epoxy embedding methods of Glauert and Kushida have been modified so as to yield rapid, reproducible, and convenient embedding methods for electron microscopy. The sections are robust and tissue damage is less than with methacrylate embedding.read more
Citations
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Journal ArticleDOI
A low-viscosity epoxy resin embedding medium for electron microscopy.
TL;DR: A low-viscosity embedding medium based on ERL-4206 is recommended for use in electron microscopy and has a long pot life of several days and infiltrates readily because of its low viscosity.
Journal ArticleDOI
The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.
TL;DR: The early stages of absorption of intravenously injected horseradish peroxidase in proximal tubules of mouse kidney were studied with a new ultrastructural cytochemical technique, which gives sharp localization and is sensitive to protein transport.
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HIGH-YIELD PREPARATION OF ISOLATED RAT LIVER PARENCHYMAL CELLS: A Biochemical and Fine Structural Study
M. N. Berry,Daniel S. Friend +1 more
TL;DR: Cytoplasmic vacuolization in a small percentage of cells and potassium loss are the only indications of cell injury detected, and the isolated cells are comparable to normal hepatic parenchymal cells in situ in appearance and function.
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Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation.
TL;DR: A postfixation in osmium tetroxide, even after long periods of storage, developed an image that—notable in the case of glutaraldehyde—was largely indistinguishable from that of tissues fixed under optimal conditions with osmia tetroxides alone.
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Junctional complexes in various epithelia
TL;DR: The tight junction is impervious to concentrated protein solutions and appears to function as a diffusion barrier or "seal," and the desmosome and probably also the zonula adhaerens may represent intercellular attachment devices.
References
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Journal ArticleDOI
Araldite as an Embedding Medium for Electron Microscopy
Audrey M. Glauert,R. H. Glauert +1 more
TL;DR: A mixture of araldite epoxy resins has been developed which is soluble in ethanol, and which yields a block of the required hardness for thin sectioning, which improves the preservation of the fine structure of a variety of specimens.
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A New Embedding Medium for Electron Microscopy
TL;DR: The introduction of n-butyl and methyl meth-acrylates as embedding media1 constituted an important development in the preparation of ultra-thin sections of biological material; but their use is attended by various major difficulties.
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Epoxy Resins in Electron Microscopy
TL;DR: A method of embedding biological specimens in araldite 502 (Ciba) has been developed for materials available in the United States, and has several features—low shrinkage and absence of specimen damage during cure, minimal compression of sections, relative absence of electron beam-induced section damage, etc.—which recommends it as a routine embedding material.