Journal ArticleDOI
Induction of root and shoot formation from root meristems ofPetunia hybrida
Reads0
Chats0
TLDR
In this paper, a procedure for the induction of root or shoot formation from root meristems of germinated seeds of Petunia hybrida was developed, where root formation was obtained on Murashige and Skoog (MS) medium supplemented with a combination of 6-benzylaminopurine (BA) (0-0.5 mg/l) and naphtaleneacetic acid (NAA) ( 0.05-2.0mg/l).Abstract:
A procedure has been developed for the induction of root or shoot formation from root meristems of germinated seeds ofPetunia hybrida. Root formation was obtained on Murashige and Skoog (MS) medium supplemented with a combination of 6-benzylaminopurine (BA) (0–0.5 mg/l) and naphtaleneacetic acid (NAA) (0.05–2.0 mg/l). Induction of predominantly shoot formation was obtained on MS medium containing the following combinations of hormones (in mg/l): 0.05–0.5 NAA and 0.25–2.0 BA. Complete plant formation was obtained after rooting of the shoots on MS medium supplemented with IAA (0–2.0 mg/l) or NAA (0-0.5 mg/l).read more
Citations
More filters
Journal ArticleDOI
Competence and determination in the process of in vitro shoot organogenesis.
M.L. Christianson,D.A. Warnick +1 more
TL;DR: The control of organogenesis by the auxin/cytokinin balance must occur between the time the tissue becomes competent and the time it is determined for shoot formation, and it is not known whether this control is a single or multiple phenomenon.
Journal ArticleDOI
Micropropagation through excised root culture of Clitoria ternatea and comparison between in vitro–regenerated plants and seedlings
TL;DR: The performance of micropropagated plants were evaluated on the basis of some physiological and biochemical parameters and compared with the in vivo-grown plants of the same age and the sodium dodecyl sulphate polyacrylamide gel electrophoresis protein profile was same between regenerated and naturally growing shoots.
Journal ArticleDOI
Light and benzylaminopurine induce changes in ultrastructure and gene expression in plastids of Petunia hybrida cell cultures.
TL;DR: Light stimulates the synthesis of polypeptides with a molecular weight of 84,000, 70,000 and 46,000 D which are encoded by cpDNA in these white culture plastids, and a gradual reduction in the number of high molecular weightPolypeptide pattern is observed at later stages of chloroplast development.
Journal ArticleDOI
Synthesis of malvidin and petunidin in pigmented tissue cultures of Petunia hybrida
TL;DR: Analysis of the pigments in petunia strain AK-5000 purple cells showed that these cells contained the same anthocyanidins (petunidin and malvidin) as found in corollas of the parental plants, suggesting an induction of Anthocyanin synthesis in these cells.
References
More filters
Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
Plantes issues de protoplastes de Pétunia
TL;DR: Now more than 500 young Petunia plants originating from mesophyll protoplasts grow in soil, some of them are flowering, the second generation is growing vigorously.
Book ChapterDOI
Plant Regeneration and Chromosome Stability in Tissue Cultures
TL;DR: Since the initial reports in 1939 independently of the successful growth and subculturing of tissue cultures of carrot and tobacco, these two dicots and a multitude of others, including soybeans, bush beans, tomato, potato, belladonna, sycamore and Jerusalem artichoke have been extensively cultured and used for a variety of studies.
Journal ArticleDOI
The isolation, culture and regeneration of leaf protoplasts in the genus Petunia
TL;DR: Differences in growth behaviour are discussed with respect to taxonomic relationship in the genus Petunia and growth requirements of the sexual hybrid between P. hybrida and P. parodii are compared to those of the parents.
Journal ArticleDOI
The bud formation in the callus cultures of Allium sativum L.
P. Havránek,F.J. Novák +1 more
TL;DR: A cytological analysis revealed the karyotypical heterogeneity of the garlic callus cells, and 2,4-D appeared to be a strong dedifferential factor blocking the mechanism of organogenesis but not histogenesis of the protracheal elements in garliccallus cultures.
Related Papers (5)
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more