Inhibition of 2,4-dichlorophenoxyacetic Acid-stimulated elongation of pea stem segments by a xyloglucan oligosaccharide.
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Xyloglucan, isolated from the soluble extracellular polysaccharides of suspension-cultured sycamore (Acer pseudoplatanus) cells, was digested with an endo-beta-1,4-glucanase purified from the culture fluid of Trichoderma viride to inhibit the elongation of etiolated pea stem segments.Abstract:
Xyloglucan, isolated from the soluble extracellular polysaccharides of suspension-cultured sycamore (Acer pseudoplatanus) cells, was digested with an endo-β-1,4-glucanase purified from the culture fluid of Trichoderma viride. A nonasaccharide-rich Bio-Gel P-2 fraction of this digest inhibited 2,4-dichlorophenoxyacetic-acid-stimulated elongation of etiolated pea stem segments. The inhibitory activity of this oligosaccharide fraction exhibited a well-defined concentration optimum between 10−2 and 10−1 micrograms per milliliter. Another fraction of the same xyloglucan digest, rich in a structurally related heptasaccharide, did not, at similar concentrations, significantly inhibit the elongation.read more
Citations
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Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth
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Isolation and characterization of plant cell walls and cell wall components
TL;DR: The methods used for isolating and characterizing the noncellulosic polysaccharides of the primary walls of suspension-cultured sycamore cells are described, which are applicable to the study of other types of cell walls.
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The Structure and Functions of Xyloglucan
TL;DR: Xyloglucan plays two very different roles in the control of cell growth: as a major building material of the wall and as a fucose-containing oligosaccharide which exerts a hormone-like anti-auxin effect on growth.
References
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Journal ArticleDOI
The Structure of Plant Cell Walls: II. The Hemicellulose of the Walls of Suspension-cultured Sycamore Cells.
TL;DR: The molecular structure, chemical properties, and biological function of the xyloglucan polysaccharide isolated from cell walls of suspension-cultured sycamore (Acer pseudoplatanus) cells are described and indicate that xylglucan is covalently linked to the pecticpolysaccharides and is noncovalently bound to the cellulose fibrils of the Sycamore cell wall.
Journal ArticleDOI
Evidence for a Requirement for Protein Synthesis for Auxin-Induced Cell Enlargement
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Turnover of cell wall polysaccharides in elongating pea stem segments.
John M. Labavitch,Peter M. Ray +1 more
TL;DR: A highly selective pattern of wall turnover processes with an even more specific influence of auxin is indicated, suggesting that indoleacetic acid induces conversion of wall xyloglucan from insoluble to water-soluble form.
Journal ArticleDOI
Regulation and in vitro translation of messenger ribonucleic acid for cellulase from auxin-treated pea epicotyls.
TL;DR: Analysis of the rates of peptide chain initiation and elongation, both in vivo and in vitro, indicated that peptideChain elongation may be rate-limiting during the lag phase of cellulase development.
Journal ArticleDOI
A general and sensitive chemical method for sequencing the glycosyl residues of complex carbohydrates
TL;DR: This paper describes a new glycosyl-sequencing technique and demonstrates the wide variety of complex carbohydrates whose structures can be ascertained by the new sequencing technique, which requires only milligram quantities of carbohydrate.