Journal ArticleDOI
Interaction of Haemophilus influenzae with Mucus, Cilia, and Respiratory Epithelium
TLDR
Fimbriation of H. influenzae increased buccal cell adherence but did not facilitate association with normal or damaged respiratory epithelium or increase epithelial damage, indicating that adhesins other than fimbriae are present.Abstract:
One nontypeable laboratory strain and five nontypeable clinical isolates of Haemophilus influenzae from sputum were investigated. Bacteria replicated from 10(4) to 10(8) cfu/ml over 24 h in an organ culture of human respiratory mucosa with only the intact mucosal surface exposed. By transmission electron microscopy, bacteria were not seen in association with normal respiratory epithelium, even after incubation for 24 h. H. influenzae infection caused patchy and occasionally confluent damage to epithelium, and the bacteria associated only with structurally damaged cells. Scanning electron microscopy revealed increased mucus, and slowed ciliary beat frequency was measured by photometry. Fimbriation of H. influenzae increased buccal cell adherence but did not facilitate association with normal or damaged respiratory epithelium or increase epithelial damage, indicating that adhesins other than fimbriae are present. Interactions with mucus, cilia, and epithelium are likely to be important in the pathogenesis of H. influenzae respiratory infections.read more
Citations
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Journal ArticleDOI
Infection and immunity
TL;DR: Mutation of the Maturase Lipop Protein Attenuates the Virulence of Streptococcus equi to a Greater Extent than Does Loss of General Lipoprotein Lipidation.
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Ciliary function and the role of cilia in clearance.
TL;DR: Mucociliary clearance is an essential part of this defence and relies on appropriate interactions between the ciliated epithelium, the height of the periciliary fluid, and mucus.
Journal ArticleDOI
Physiologic and hypertonic saline solutions impair ciliary activity in vitro
TL;DR: In vitro effects of saline solutions in different concentrations on ciliary beat frequency suggest that mucolytic effects induced by hyperosmolarity should be attained preferably with hypertonic saline 7% in patients with cystic fibrosis or asthma.
Journal ArticleDOI
Diagnostic Testing of Patients Suspected of Primary Ciliary Dyskinesia
TL;DR: The use of CBF alone to screen which biopsies should have EM will result in a significant number of missed diagnoses and ciliary beat pattern analysis is a more sensitive and specific test for PCD with higher PPV and NPV.
Journal ArticleDOI
Paracytosis of Haemophilus influenzae through cell layers of NCI-H292 lung epithelial cells.
TL;DR: It is concluded that H. influenzae passes through viable cell layers of the human lung epithelial cell line NCI-H292 by paracytosis, requiring bacterial protein synthesis.
References
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Journal ArticleDOI
Effect of bacterial products on human ciliary function in vitro.
TL;DR: It is concluded that Ps aeruginosa and H influenzae release a factor (or factors) which causes slowing of human nasal cilia in vitro, and the role of this factor in the pathogenesis of infection is discussed.
Journal ArticleDOI
Interaction of Nontypable Haemophilus influenzae with Human Respiratory Mucosa In Vitro
Robert C. Read,Robert Wilson,Andrew Rutman,Valerie J. Lund,H. C. Todd,Anthony P. R. Brain,Peter K. Jeffery,Peter J. Cole +7 more
TL;DR: Fimbriation of H. influenzae increased buccal cell adherence but did not facilitate association with normal respiratory epithelium and failed to increase epithelial damage or association with damaged cells.
Journal ArticleDOI
Adherence of Pseudomonas aeruginosa to human tracheobronchial mucin.
S Vishwanath,R Ramphal +1 more
TL;DR: It is inferred that there may be specific sites on human tracheobronchial mucin which facilitate this preferential binding of P. aeruginosa, and similar studies with strains of Escherichia coli and Klebsiella pneumoniae indicated a relative lack of binding of these bacteria to mucin.
Journal ArticleDOI
Frequency of fimbriation of nontypable Haemophilus influenzae and its ability to adhere to chinchilla and human respiratory epithelium.
Lauren O. Bakaletz,B. M. Tallan,Toni M. Hoepf,Thomas F. DeMaria,Herbert G. Birck,David J. Lim +5 more
TL;DR: The level or degree of fimbriation did not correlate with either site of isolation, biotype, strength of hemagglutination reaction, or type of effusion present in the ear, and these appendages appear to be quite different from those described for type b H. influenzae.
Journal ArticleDOI
Effect of a Toxin Produced by Haemophilus influenzae on Ciliated Respiratory Epithelium
TL;DR: Tracheal organ cultures from the human fetus, the chick embryo, and several rodents were used in a study of the effect of Haemophilus influenzae on respiratory ciliated epithelial cells, resulting in ciliostasis after various periods of time.