Isolation and characterization of acyclovir-resistant mutants of herpes simplex virus.
TLDR
One mutant is described (SC16 R9C2) which is resistant in biochemically transformed cells which express HSV TK, which suggests that its resistance resides at a level other than TK.Abstract:Â
Mutants of HSV which are resistant to acyclovir (acycloguanosine) have been isolated following serial passages of several herpes simplex virus (HSV) strains in the presence of the drug. The majority of the mutants isolated are defective in induction of thymidine kinase (TK) and this is consistent with the observation that independently isolated TK- viruses are naturally resistant to ACV. One mutant is described (SC16 R9C2) which is resistant in biochemically transformed cells which express HSV TK. This suggests that its resistance resides at a level other than TK. It is also resistant to phosphonoacetic acid, suggesting that the DNA polymerase locus may be involved. A further mutant is described [Cl (101) P2C5] which induces normal levels of TK, although the nature of resistance of this virus is not yet elucidated.read more
Citations
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Journal ArticleDOI
Thymidine kinase-negative herpes simplex virus mutants establish latency in mouse trigeminal ganglia but do not reactivate
Donald M. Coen,Magdalena Kosz-Vnenchak,Jennie G. Jacobson,David A. Leib,Connie L. Bogard,Priscilla A. Schaffer,Kenneth L. Tyler,David M. Knipe +7 more
TL;DR: It is suggested that acyclovir-resistant viruses could establish latent infections in clinical settings and have implications for the use of genetically engineered herpesviruses to deliver foreign genes to neurons.
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Herpes simplex virus DNA replication.
Paul E. Boehmer,I. R. Lehman +1 more
TL;DR: DNA replication very likely proceeds in two phases: an initial phase of theta replication, initiated at one or more of the origins, followed by a rolling-circle mode of replication, which generates concatemers that are cleaved and packaged into infectious viral particles.
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Herpes Simplex Virus Resistant to Acyclovir: A Study in a Tertiary Care Center
Janet A. Englund,Mark E. Zimmerman,Ella M. Swierkosz,Jesse L. Goodman,David R. Scholl,Henry H. Balfour +5 more
TL;DR: Herpes simplex virus resistant to acyclovir arises relatively frequently in immunocompromised patients and may cause serious disease and the importance of resistant isolates in hospitalized patients is highlighted.
Patent
Eukaryotic layered vector initiation systems
Thomas W. Dubensky,John M. Polo,Carlos E. Ibanez,Stephen M. W. Chang,Douglas J. Jolly,David A. Driver,Barbara A. Belli +6 more
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A new nucleoside analog, 9-[[2-hydroxy-1-(hydroxymethyl)ethoxyl]methyl]guanine, highly active in vitro against herpes simplex virus types 1 and 2.
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References
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Effect of Acycloguanosine Treatment on Acute and Latent Herpes Simplex Infections in Mice
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