Linkage Groups in Bacillus pumilus Determined by Bacteriophage PBS1-Mediated Transduction
Paul S. Lovett,Frank E. Young +1 more
TLDR
Two linkage groups were established in Bacillus pumilus NRRL B-3275 by bacteriophage PBS1 transduction and contains the auxotrophic markers gly, his, and met.Abstract:
Two linkage groups were established in Bacillus pumilus NRRL B-3275 by bacteriophage PBS1 transduction. Group L contains the auxotrophic markers gly, his, and met. Group M contains the markers argO, met, ura, and cys.read more
Citations
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Journal ArticleDOI
Bacteriophages of Bacillus subtilis.
H E Hemphill,H R Whiteley +1 more
TL;DR: This article corrects the article on p. 298 in vol.
Journal ArticleDOI
Intergenotic transformation of the Bacillus subtilis genospecies
Gary A. Wilson,Frank E. Young +1 more
TL;DR: A multiple auxotrophic derivative of Bacillus subtilis 168 (strain BR151 carrying lys-3, trpC2, metB10) was transformed with deoxyribonucleic acid (DNA) isolated from B. subtil is 168 to prepare donor DNA, and adverse side effects can occur after incorporation of a segment of foreign DNA.
Journal ArticleDOI
PBP1: A flagella specific bacteriophage mediating transduction in Bacillus pumilus
TL;DR: It is concluded that PBP1 interacts with the bacterial flagellum during the adsorption process and mediates generalized transduction in BpB1 at a frequency on the order of 10−8 transductants per plaque-forming unit.
Journal ArticleDOI
Plasmid in Bacillus pumilus and the enhanced sporulation of plasmid-negative variants.
TL;DR: A relationship appears to exist between the oligosporogenic properties of NRS576 and the presence of the 576 plasmid, which is known to form spores during incubation in liquid AK sporulation medium.
Journal ArticleDOI
Comparative Zone Electrophoresis of Enzymes in the Genus Bacillus
TL;DR: Extracts from 88 strains representing a wide variety of Bacillus species were examined by zone electrophoresis, followed by specific stains for each of 11 enzyme activities, and were also analyzed for deoxyribonucleic acid (DNA) buoyant density.
References
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Journal ArticleDOI
Genetic mapping in Bacillus subtilis.
TL;DR: A map of the Bacillus subtilis genome has been constructed from the results of density transfer experiments carried out in strain W23, and from genetic linkage data obtained using DNA-mediated transformation and phage PBS1-mediated transduction in strain 168.
Journal ArticleDOI
Identification of Bacillus subtilis NRRL B-3275 as a Strain of Bacillus pumilus
Paul S. Lovett,Frank E. Young +1 more
TL;DR: The biotin requirement of B-3275, its inability to hydrolyze starch, and its failure to reduce nitrate indicate that the organism is more closely related to the B. pumilus strains than to those of B. subtilis.
Journal ArticleDOI
Genetic Analysis in Bacillus pumilus by PBS1-Mediated Transduction
Paul S. Lovett,Frank E. Young +1 more
TL;DR: Transduction of any of the above cited markers in BpB1 to prototrophy with PBS1 propagated on B. subtilis 168 could not be demonstrated, and the nature and relative order of the markers within each linkage group suggest that the arrangement of genes in these areas of the chromosome of B pumilus is similar to the arrangementOf phenotypically comparable genes in two linkage groups (defined by PBS1 transduction).
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Genetic Analysis in Bacillus pumilus by PBS1-Mediated Transduction
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Identification of Bacillus subtilis NRRL B-3275 as a Strain of Bacillus pumilus
Paul S. Lovett,Frank E. Young +1 more