Lysis of Bacterial Cell Walls by an Enzyme Isolated from a Myxobacter
J. C. Ensign,Ralph S. Wolfe +1 more
TLDR
An exoenzyme which lyses intact cells, heat-killed cells, and cell walls of Arthrobacter crystallopoietes was purified 60-fold from the growth liquor obtained from a myxobacter (strain AL-1).Abstract:
Ensign, J. C. (University of Illinois, Urbana), and R. S. Wolfe. Lysis of bacterial cell walls by an enzyme isolated from a myxobacter. J. Bacteriol. 90:395–402. 1965.—An exoenzyme which lyses intact cells, heat-killed cells, and cell walls of Arthrobacter crystallopoietes was purified 60-fold from the growth liquor obtained from a myxobacter (strain AL-1). The lytic enzyme was produced during growth of the organism in a number of complex media, the maximal amount of enzyme being produced in yeast extract broth. The purified enzyme lysed at different rates a number of gram-positive bacteria. With the exception of Rhodospirillum rubrum, Spirillum itersonii, and S. serpens, the gram-negative bacteria tested were not attacked.read more
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Lysobacter, a New Genus of Nonfruiting, Gliding Bacteria with a High Base Ratio
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References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Lysostaphin: a new bacteriolytic agent for the staphylococcus.
C. A. Schindler,V. T. Schuhardt +1 more
TL;DR: A chronology of key events and publications: 1.
Journal ArticleDOI
Nutritional requirements for vegetative growth of myxococcus xanthus
TL;DR: By use of a dispersed-growing strain of Myxococcus xanthus, the nutritional requirements for vegetative growth have been defined and, with the exception of glycogen, a variety of organic materials do not significantly stimulate growth.
Journal ArticleDOI
Structure of the cell wall of staphylococcus aureus, strain copenhagen. i. preparation of fragments by enzymatic hydrolysis.
Journal ArticleDOI
Studies on Nonfruiting Myxobacteria: I. Cytophaga johnsonae, n.sp., a Chitin-decomposing Myxobacterium.
TL;DR: The concept of myxobacteria as a microbial assemblage characterized by a complex life cycle involving a unicellular phase of vegetative proliferation and a communal phase of fructification was clearly expressed by Thaxter (1892) in the first detailed publication dealing with the group.
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