Patent
Method and apparatus for imaging a sample on a device
TLDR
In this article, a support synthesized with polymer sequences at known locations according to the methods disclosed in U.S. Pat. No. 5,143,854 and PCT WO 92/10092 or others, can be detected by exposing selected regions of sample 1500 to radiation from a source 1100 and detecting the emission therefrom, and repeating the steps of exposition and detection until the sample is completely examined.Abstract:
Labeled targets on a support synthesized with polymer sequences at known locations according to the methods disclosed in U.S. Pat. No. 5,143,854 and PCT WO 92/10092 or others, can be detected by exposing selected regions of sample 1500 to radiation from a source 1100 and detecting the emission therefrom, and repeating the steps of exposition and detection until the sample is completely examined.read more
Citations
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PatentDOI
Expression monitoring by hybridization to high density oligonucleotide arrays
TL;DR: In this article, the authors proposed a method for monitoring the expression levels of a multiplicity of genes by hybridizing a nucleic acid sample to a high density array of oligonucleotide probes and quantifying the hybridized nucleic acids in the array.
Patent
Methods for fabricating microarrays of biological samples
TL;DR: In this paper, a method and apparatus for forming microarrays of biological samples on a support is described, which involves dispensing a known volume of a reagent at each selected array position, by tapping a capillary dispenser on the support under conditions effective to draw a defined volume of liquid onto the support.
PatentDOI
Sequence-specific inhibition of small RNA function
TL;DR: The RISC inactivators of the present invention enable a variety of methods for identifying and characterizing miRNAs and siRNAs, RISC-associated factors, and agents capable of modulating RNA silencing.
Patent
Methods and apparatuses for analyzing polynucleotide sequences
TL;DR: In this article, a high speed, high throughput analysis of polynucleotide sequences, and apparatuses with which to carry out the methods are provided in the invention of the algorithm.
Patent
Nucleic acid analysis techniques
David J. Lockhart,Mark S. Chee,Kevin L. Gunderson,Chao-Qiang Lai,Lisa Wodicka,Maureen T. Cronin,Danny Lee,Huu M. Tran,Hajime Matsuzaki,Glenn H. McGall,Anthony D. Barone +10 more
TL;DR: In this article, a simplified method for identifying differences in nucleic acid abundances (e.g., expression levels) between two or more samples is presented. But the method is limited to the case where the sequence and location of each different probe is known.
References
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Patent
Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
TL;DR: In this paper, a polypeptide array can be synthesized on a substrate by attaching photoremovable groups to the surface of a substrate, exposing selected regions of the substrate to light to activate those regions, attaching an amino acid monomer with a photoregressive group to the activated regions, and repeating the steps of activation and attachment until the desired length and sequences are synthesized.
Patent
Very large scale immobilized polymer synthesis
TL;DR: In this paper, a method and apparatus for preparation of a substrate containing a plurality of sequences is described, where a set of photoremovable groups are attached to a surface of the substrate and selected regions of the surface are exposed to light so as to activate the selected areas.
Patent
Method and apparatus for conducting an array of chemical reactions on a support surface
TL;DR: In this paper, the authors present a method for making arrays of functionalized binding sites on a support surface for sequencing oligonucleotides and for identifying the amino acid sequence of peptides that bind to biologically active macromolecules.
Patent
Arrays of nucleic acid probes on biological chips
Mark S. Chee,Maureen T. Cronin,Stephen P. A. Fodor,Thomas R. Gingeras,Xiaohua C. Huang,Earl Hubbell,Robert J. Lipshutz,Peter E. Lobban,Charles Garrett Miyada,Macdonald S. Morris,Nila Shah,Edward L. Sheldon +11 more
TL;DR: In this paper, the authors proposed chips of immobilized probes for comparing a reference polynucleotide sequence of known sequence with a target sequence showing substantial similarity with the reference sequence, but differing in the presence of e.g., mutations.
Patent
Capillary flow device
Robert S. Hillman,Michael E. Cobb,Jimmy D Allen,Ian Gibbons,Vladimir E Ostoich,Laura J. Winfrey +5 more
TL;DR: In this paper, a fabrication technique particularly suited for forming internal chambers in plastic devices is described along with various control devices for use with the device, where the resulting agglutination particles may provide for changes in flow rate, light patterns of a flowing medium, or light absorption or scattering.