Method for efficiently producing hydroxysteroid dehydrogenase with testosterone comamonas

TLDR: In this article, a method for efficiently producing hydroxysteroid dehydrogenase with testosterone comamonas was proposed, which comprises steps of bacteria culture, seeding tank culture, fermentation tank culture and the like.

Abstract: The invention relates to a method for efficiently producing hydroxysteroid dehydrogenase with testosterone comamonas. The method comprises steps of bacteria culture, seeding tank culture, fermentation tank culture and the like, and can produce hydroxysteroid dehydrogenase efficiently. The hydroxysteroid dehydrogenase has remarkable effects on crops in preventing disease, promoting growth and increasing yield. Furthermore, if being used as a novel fodder enzyme preparation in cultivation in stock farming, the hydroxysteroid dehydrogenase can effectively promote pigs and broiler chickens to grow, improves the laying rate of laying hens, reduces disease of beasts and birds, and has obvious effect in promoting growth. According to the method, viable bacteria in per milliliter of fermentation liquor is improved to be 30 billion from 0.1 billion, the enzyme activity in per gram of dry powder is improved to be 300 thousand from 1 thousand, so that the product cost is greatly lowered, and the method can be used for industrial production.