Microbial community structure and pH response in relation to soil organic matter quality in wood-ash fertilized, clear-cut or burned coniferous forest soils

TLDR: In this article, the effects of treatments on microbial biomass and community structure in coniferous forests were investigated using the Humus phospholipid fatty acid (PLFA) analysis, which showed that the microbial biomass decreased significantly due to the highest rate of wood-ash fertilization, clear-cutting, and the two different fire treatments when compared to control amounts.

Abstract: Humus phospholipid fatty acid (PLFA) analysis was used in clear-cut, wood-ash fertilized (amounts applied: 1000, 2500, and 5000 kg ha−1), or prescribed burned (both in standing and clear-cut) coniferous forests to study the effects of treatments on microbial biomass and community structure. The microbial biomass (total PLFAs) decreased significantly due to the highest rate of wood-ash fertilization, clear-cutting, and the two different fire treatments when compared to control amounts. Fungi appeared more seriously reduced by these treatments than bacteria, as revealed by a decreased index of fungal:bacterial PLFAs when compared to the controls. The community structure was evaluated using the PLFA pattern. The largest treatment effect was due to burning in both areas studied, which resulted in increases in 16:1ω5 and proportional decreases in 18:2ω6. Clear-cutting and the different amounts of ash application resulted in similar changes in the PLFA pattern to the burning treatments, but these were less pronounced. Attempts to correlate the changes in the PLFA pattern to soil pH, bacterial pH response patterns (measured using thymidine incorporation), or substrate quality (measured using IR spectroscopy) were only partly successful. Instead, we hypothesize that the changes in the PLFA pattern of the soil organisms were related to an altered substrate quantity, that is the availability of substrates after the treatments.