Micropropagation of Carob tree (Ceratonia siliqua L.) by cotyledonary buds

TLDR: In vitro micropropagation of Carob tree (Ceratonia siliqua L.) by cotyledonary buds taken from 13 day old seedlings was attempted on Woody Plant Medium (WPM) supplemented with microelements and Murashige and MS vitamins.

Abstract: In vitro micropropagation of Carob tree (Ceratonia siliqua L.) by cotyledonary buds taken from 13 day old seedlings was attempted on Woody Plant Medium (WPM) supplemented with microelements and Murashige and Skoog (MS) vitamins. Comparison of the effect of four cytokinines (benzylaminopurine, zeatin, kinetinand 2-iP) has shown that seedling growth was stimulated by zeatin at 1 mg/L, while organogenesis was better under BAP or zeatin at 1 mg/L. The effect of four auxins (AIA, AIB, ANA and 2,4-D) at low concentrations (0.1 mg/L) combined with BAP at 0.5 mg/L has shown that Naphthalene Acetic Acid (NAA) stimulates better stem formation and growth. Moreover, combination of Gibberellic Acid (GA3) at different concentrations with BAP at 0.5 mg/L did not show a significant effect on growth. Shoot multiplication was stimulated by medium concentrations of BAP (0.5 - 1 mg/L) alone or with GA3 at 0.2 mg/L. Among the auxins tested during rooting, Indol-3-Butyric Acid (IBA) (2 mg/L) was found to be the most efficient for root formation.