Morphology of Ureaplasma urealyticum (T-mycoplasma) organisms and colonies.
TLDR
The addition of both urea and putrescine to the growth medium and incubation in a humidified CO2 atmosphere are recommended for improved growth and formation of fried-egg colonies of U. ureaplyticum on agar.Abstract:
The morphology of Ureaplasm urealyticum in broth cultures was studied by phase-contrast microscopy. Most organisms appeared singly or in pairs. Long filaments and long chains of cocci, common in classical mycoplasma cultures, were not observed. On solid medium, U. urealyticum produced "fried-egg" colonies which developed according to the scheme suggested by Razin and Oliver (J. Gen. Microbiol., 1961) for the morphogenesis of the classical mycoplasma colonies. The formation of the peripheral zone of the colonies followed that of the central zone only when growth conditions were adequate, Hence, the appearance of peripheral zones, and consequently the larger colony size, can be taken as an indicator of improved growth conditions. Incubation in an atmosphere of 100% CO2 resulted in significantly larger colonies than in an atmosphere of N2, O2, or air. CO2 acts as a buffer, keeping the pH at the optimal range for Ureaplasma growth (pH 6.0 to 6.5) in the presence of the ammonia produced from the urea hydrolyzed by the organisms. The addition to the medium of 0.01 M urea together with 0.01 M putrescine enabled better growth than with urea alone. Small amounts of phosphate improved growth in an atmosphere of CO2, apparently fulfilling a nutritional role. Under nitrogen, higher phosphate concentrations were required for good growth, apparently serving as a buffer as well as a nutrient. Sodium chloride and sucrose which had been added to increase the tonicity of the medium inhibited growth above 0.1 M. An increase in the agar concentration above 2% resulted in decreased colony size. Likewise, prolonged drying of the agar plates caused a marked decrease in colony size, mostly affecting the peripheral zone. The addition of both urea and putrescine to the growth medium and incubation in a humidified CO2 atmosphere are recommended for improved growth and formation of fried-egg colonies of U. ureaplyticum on agar. It must be emphasized that these experiments were carried out with a laboratory-adapted strain.read more
Citations
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Molecular Cloning and Characterization of Three β-Defensins from Canine Testes
TL;DR: In this article, the authors cloned the full-length cDNA of three canine β-defensin isoforms (cBD-1, -2, and -3) from canine testicular tissues.
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Enhancement of Ureaplasma urealyticum growth on a differential agar medium (A7B) by a polyamine, putrescine.
M C Shepard,R S Combs +1 more
TL;DR: Growth of Ureaplasma urealyticum isolates from clinical exudates and urine specimens was significantly improved by supplementation of a Urea Plasma differential agar medium by putrescine, designated medium A7B.
References
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Journal ArticleDOI
Color Test for the Measurement of Antibody to T-Strain Mycoplasmas
TL;DR: Antibody to mycoplasma strain T-960 in human sera was demonstrated with the metabolic inhibition technique, and the T-strain organism was shown to be serologically distinct from the recognized large-colony myCoplasmas.
Journal ArticleDOI
Ureaplasma urealyticum gen. nov., sp. nov.: Proposed Nomenclature for the Human T (T-Strain) Mycoplasmas
M. C. Shepard,C. D. Lunceford,D. K. Ford,Robert H. Purcell,D. Taylor-Robinson,Shmuel Razin,Finn T. Black +6 more
TL;DR: It is reasonable to propose establishing a new, separate genus in the family Mycoplasmataceae in which to classify the T mycoplAsmas isolated from man and lower animals, and the name Ureaplasma urealyticum is proposed for this new species.
Journal ArticleDOI
Occurrence of Urease in T Strains of Mycoplasma
TL;DR: T strains were cultivated in a serum-free fluid medium containing urea as the only added metabolite and nitrogen source, and this biochemical marker can be employed as an aid in the detection and identification of T strains of Mycoplasma (urease color test) and in distinguishing T strains from other members of the human MyCoplasma group.
Journal ArticleDOI
Influence of Urea on the Growth of T-Strain Mycoplasmas
Denys K. Ford,Joyce Macdonald +1 more
TL;DR: Stock T-strain cultures are optimally maintained in continuous culture if the routine medium at pH 6.0 is supplemented with 0.05% urea and 0.002% phenol red, but an incubation temperature of 30 C is preferable to 37 C for subculture at 24-hr intervals.
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