Journal ArticleDOI
Nature of soil organic phosphorus: an assessment of peak assignments in the diester region of 31P NMR spectra
TLDR
In this paper, peak assignments in 31 P NMR spectra of alkaline soil extracts were analyzed and it was shown that the resonance at 0.5-1.9ppm was caused by DNA-P and the other diesters were represented by teichoic acids.Abstract:
This study involved a critical assessment of peak assignments in 31 P NMR spectra of alkaline soil extracts and included 31 P NMR spectroscopy of (1) 500 mM NaOH solutions of RNA, DNA, and lecithin; (2) 50 mM H 2 SO 4 , 500 mM NaHCO 3 (pH 8.5), and 100 mM NaOH extracts from Pseudomonas putida (Gram-negative bacterium), Bacillus subtilis (Gram-positive bacterium), Penicillium citrinum (a fungus), Aspergillus niger (a fungus), and leaves of Betula pubescens , Picea abies , and Pinus cembra ; (3) 100 mM NaOH solutions of evaporated methanol–chloroform extracts from B. subtilis , P. putida , B. pubescens leaves, and from organic and mineral soil horizons; (4) 100 mM NaOH and 50 mM H 2 SO 4 extracts from delipidized bacterial cells, and 100 mM NaOH extracts from delipidized birch leaves and soil samples. Results showed that the resonance at 0 ppm, previously assigned to phospholipids and nucleic acids, was caused by DNA-P. Resonances of phospholipids of plant and microbial origin were observed at about 1.5–1.7 and 0.6–0.7 ppm, in regions previously assigned to teichoic acid-P by various authors. Non-lipidic compounds extracted from B. subtilis and resonating at 1.9 ppm probably were represented by teichoic acids. 31 P NMR spectroscopy cannot differentiate signals derived from P of phospholipids and non-lipidic compounds in the low field of the diester region of spectra of alkaline extracts from soils, while quantitative differentiation of DNA-P and the other diesters seems quite a simple task. An unknown resonance at −1.5 ppm remained unidentified. The real concentrations of diester-P in soils can be considerably underestimated and those of monoester-P overestimated, when analyzed in alkaline extracts, because of almost complete hydrolysis of RNA and partial hydrolysis of phospholipids to monoesters.read more
Citations
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Journal ArticleDOI
Phosphorus-31 nuclear magnetic resonance spectral assignments of phosphorus compounds in soil NaOH–EDTA extracts
TL;DR: In this paper, a solution 31P NMR chemical shifts of model P compounds, including inorganic phosphates, orthophosphate monoesters and diesters, phosphonates, and organic polyphosphates, were determined in a standardized soil P extractant (0.25 M NaOH and 0.05 M EDTA).
Journal ArticleDOI
Extraction of soil organic phosphorus.
TL;DR: This work critically review the extraction of soil organic phosphorus, including procedures for quantification, speciation, and assessment of biological availability, including a single-step procedure involving sodium hydroxide and EDTA.
Journal ArticleDOI
Characterizing phosphorus in environmental and agricultural samples by 31P nuclear magnetic resonance spectroscopy
TL;DR: The requirements for a successful solid-state or solution (31)P-NMR experiment are described, including experimental set-up, sample preparation, extractants, experimental conditions, and post-experimental processing.
Journal ArticleDOI
Soil Organic Phosphorus Transformations During Pedogenesis
TL;DR: In this paper, the chemical nature of soil organic phosphorus along a 120,000-year postglacial chronosequence at Franz Josef, New Zealand has been investigated.
References
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Journal ArticleDOI
a Comparison of Soil Extraction Procedures for 31P NMR Spectroscopy
TL;DR: In this paper, the effect of extractants on phosphorus determination by 31 P NMR spectroscopy was examined using five forest floor samples using five extractants: 0.25 M NaOH, 1.6 soil to Chelex in water, 1:6 soil-to-Chelex in NaOH and 1:1 mix of 0.5 M Naoh and O.1 M EDTA.
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Soil phosphorus characterisation by 31p nuclear magnetic resonance
R. H. Newman,K. R. Tate +1 more
TL;DR: In this paper, five New Zealand topsoils, including three sampled under introduced pasture and two in native tussock grasslands, were extracted with 0.5 M NaOH, and the different classes of phosphorus compounds.
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Nature and distribution of soil phosphorus as revealed by a sequential extraction method followed by 31P nuclear magnetic resonance analysis
TL;DR: In this article, a sequential extraction procedure followed by 31P nuclear magnetic resonance (NMR) analysis of the soil phosphorus (P) forms present was used to determine 80% of the total organic P (as determined by ignition) was removed from the soil.
Journal ArticleDOI
Phosphorus-31 nuclear magnetic resonance studies of wild-type and glycolytic pathway mutants of Saccharomyces cerevisiae.
TL;DR: High-resolution phosphorus-31 nuclear magnetic resonance (31P NMR) spectra of wild-type and mutant strains of Saccharomyces cerevisiae were observed and each mutant strain was found to accumulate characteristic sugar phosphates when glucose was added to the cell suspension.