Patent
On-line product identification in a chromatography effluent by subtraction.
Noubar B. Afeyan,Fred E. Regnier +1 more
TLDR
Disclosed as discussed by the authors is a method and apparatus for the rapid identification of a solute of interest in an effluent stream, which involves separation of the solutes in the effluent and identification of the particular solutes of interest by its selective subtraction.Abstract:
Disclosed is a method and apparatus for the rapid identification of a solute of interest in an effluent stream (14). The method involves separation of the solutes in the effluent (14) and identification of a particular solute of interest by its selective subtraction from the effluent stream (14). The effluent stream (14) from a first system (12) capable of partitioning the components of the mixture is passed through a detector (16) to produce a first output (18) which describes the temporal and/or spacial sequence of the mixture components exiting the first system (12). The effluent stream (14) is then passed through a second system (20) capable of selectively extracting a solute of interest from said effluent stream (14) and is then passed through a detector (16).read more
Citations
More filters
Patent
Monitoring amplification of DNA during PCR
TL;DR: In this article, methods for quantitating amplified DNA and determining its purity are carried out by analysis of melting and reannealing curves, achieved with rapid thermal cycling and use of double stranded DNA dyes or specific hybridization probes.
Patent
On-line DNA analysis system with rapid thermal cycling
Harold Swerdlow,Carl T. Wittwer +1 more
TL;DR: In this paper, a capillary electrophoresis column with an inlet and outlet end, a means of injection, and means of applying a high voltage to cause the differential migration of species of interest through the capillary column.
Patent
Container for carrying out and monitoring biological processes
Carl T. Wittwer,Kirk M. Ririe +1 more
TL;DR: In this paper, a thermal cycling method and device for DNA polymerase chain reaction (PCR) has been described, which can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures.
Patent
System and method for fluorescence monitoring
TL;DR: In this paper, a thermal cycling method and device is described, which comprises a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures such as the DNA polymerase chain reaction.
Patent
Monitoring hybridization during PCR using SYBR Green I
TL;DR: In this article, a method and kits for monitoring a nucleic acid during amplification was presented, where a double-stranded binding dye was used to produce a fluorescent signal related to the amount of nucleic acids present in a sample.
References
More filters
Book
Introduction to modern liquid chromatography
TL;DR: A short history of HPLC can be found in this paper, where the authors present a detailed overview of the current state of the art in HPLC and its application in the literature.
Journal ArticleDOI
Flow-through particles for the high-performance liquid chromatographic separation of biomolecules: perfusion chromatography.
Noubar B. Afeyan,Neal F. Gordon,István Mazsaroff,Laszlo Varady,S.P. Fulton,Yi Yang,Fred E. Regnier +6 more
TL;DR: It is shown that the rate of solute transport is strongly coupled to mobile phase Velocity such that bandspreading, resolution of proteins and dynamic loading capacity are unaffected by increases in mobile phase velocity up to several thousand centimeters per hour.
Patent
Chromatographic method and means
TL;DR: In this paper, a novel packing material for liquid chromatographic columns, having a maximum pore size around 80 angstroms, a hydrophobic coating on the inner surfaces adapted to absorb hydrophilic analytes, and a pH-sensitive coating on outer surfaces adapted for rejecting proteins in the analyte sample.
Patent
Column chromatographic column apparatus with switching capability
TL;DR: In this paper, a dual column chromatographic apparatus with column switching capability was presented for the separation of a multicomponent sample into one or more components, which is useful in any pressurized fluid chromatography system.
Patent
Chromatographic apparatus and method
TL;DR: In this paper, an apparatus and method for increasing the throughput of a chromatographic system by passing several samples in seriatim through tandemly connected first and second columns is presented, where the eluant from the first column is sampled and fractions of each of the samples are passed through the second column for further separation.