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Journal ArticleDOI

PCR detection of the two 'Candidatus' liberobacter species associated with greening disease of citrus

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TLDR
A PCR method to detect the two liberobacter species in citrus trees by amplification of a 1160 bp fragment of their 16S rDNA is described.
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This article is published in Molecular and Cellular Probes.The article was published on 1996-02-01. It has received 369 citations till now. The article focuses on the topics: Candidatus Liberibacter & Candidatus.

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Citations
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Huanglongbing: a destructive, newly-emerging, century-old disease of citrus.

TL;DR: Control of huanglongbing (HLB), a destructive disease of citrus that represents a major threat to the world citrus industry, is preventive and largely based on inoculum elimination by removal of infected trees and chemical treatments against vectors.
Journal ArticleDOI

Asian citrus psyllids (sternorrhyncha: psyllidae) and greening disease of citrus: a literature review and assessment of risk in florida

TL;DR: Management of citrus greening disease is difficult and requires an integrated approach including use of clean stock, elimination of inoculum via voluntary and regulatory means, use of pesticides to control psyllid vectors in the citrus crop, and biological control of psyllID vectors in non-crop reservoirs.
Journal ArticleDOI

Quantitative real-time PCR for detection and identification of Candidatus Liberibacter species associated with citrus huanglongbing.

TL;DR: The authors' rapid, sensitive and specific TaqMan PCR assay for the detection, identification and quantification of Ca.
Journal ArticleDOI

A new Huanglongbing Species, "Candidatus Liberibacter psyllaurous," found to infect tomato and potato, is vectored by the psyllid Bactericera cockerelli (Sulc).

TL;DR: A new huanglongbing (HLB) species is genetically characterized, and the bacterium is designated “Candidatus Liberibacter psyllaurous,” potentially resulting in “psyllid yellowing.”

Huanglongbing: a destructive, newly-emerging, century-old disease of citrus 1

TL;DR: Huanglongbing (HLB), a destructive disease of citrus that represents a major threat to the world citrus industry, and is slowly invading new citrusgrowing areas, is given.
References
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Journal ArticleDOI

Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase

TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
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The phloem-limited bacterium of greening disease of citrus is a member of the α subdivision of the Proteobacteria

TL;DR: Comparisons with sequences of 16S rDNAs obtained from the GenBank data base revealed that the two citrus greening disease BLOs belong to the alpha subdivision of the class Proteobacteria, and it is proposed that this new group of bacteria is called "liberobacter".
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Citrus greening disease.

TL;DR: This review aims to present an overview of greening worldwide, and lists 556 papers in his 1990 bibliography, and the present author found a further 86, although many do not advance the understanding of the disease.
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Taxonomic notes: a proposal for recording the properties of putative taxa of procaryotes

TL;DR: This note is presented in advance of discussions by the International Committee on Systematic Bacteriology at the forthcoming congress (July 1994) so that there may be discussions and reaction by colleagues.
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