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Penta- and disaccharide lipid formation by Nocardia corynebacteroides grown on n-alkanes

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TLDR
Both 1H and 13C nuclear magnetic resonance spectroscopic and elemental analysis studies led to the identification of one novel pentasaccharide lipid and two trehalose corynomycolates, which showed significant surface and interfacial active properties.
Abstract
Nocardia corynebacteroides SM1 synthesized di- and pentasaccharide lipids when grown on n-alkanes, especially under nitrogen limitation. Optimum conditions for their formation were pH 6.4–6.8, NaNO3 as nitrogen source and yeast extract supplementation of the nutrient. A study of the time course of whole glycolipid production was carried out in a 20-l bioreactor. After extraction of the culture broth with organic solvents three main components could be isolated. Both 1H and 13C nuclear magnetic resonance spectroscopic and elemental analysis studies led to the identification of one novel pentasaccharide lipid and two trehalose corynomycolates. The oligosaccharide lipid showed significant surface and interfacial active properties.

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Citations
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Journal ArticleDOI

Microbial production of surfactants and their commercial potential.

TL;DR: Biosurfactants are more effective, selective, environmentally friendly, and stable than many synthetic surfactants, and the most promising applications are cleaning of oil-contaminated tankers, oil spill management, transportation of heavy crude oil, enhanced oil recovery, recovery of crude oil from sludge, and bioremediation of sites contaminated with hydrocarbons, heavy metals, and other pollutants.
Journal ArticleDOI

Nuclear Magnetic Resonance

J. Lee
- 01 May 1968 - 
TL;DR: Emsley and Waugh as mentioned in this paper published the Guide to the NMR Empirical Method A Workbook, which is based on the Bible jun.. Pp. xi + 305.
Journal ArticleDOI

Surface-active compounds from microorganisms.

TL;DR: Recent progress in the genetic and biochemical analysis of biosurfactant synthesis as well as the current status of fermentation technologies are discussed.
Journal ArticleDOI

Formation and physiological role of biosurfactants produced by hydrocarbon-utilizing microorganisms. Biosurfactants in hydrocarbon utilization.

TL;DR: A more differentiated view of microbial production of surface-active compounds is proposed, which suggests that biosurfactants should not only be regarded as prerequisites of hydrocarbon uptake, but also as secondary metabolic products.
Journal ArticleDOI

Microbial surfactant-enhanced mineral oil recovery under laboratory conditions

TL;DR: Biosurfactant produced by the promising bacterial isolates have been found to be effective in the recovery of crude oil from saturated column under laboratory conditions.
References
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Journal ArticleDOI

A rapid and precise method for the determination of urea

TL;DR: In comparison with other methods in current use, this method has distinct advantages in sensitivity, simplicity, and precision, thus economizing in time, sample volume, reagents, and equipment.
Journal ArticleDOI

Nuclear Magnetic Resonance

J. Lee
- 01 May 1968 - 
TL;DR: Emsley and Waugh as mentioned in this paper published the Guide to the NMR Empirical Method A Workbook, which is based on the Bible jun.. Pp. xi + 305.
Journal ArticleDOI

Microbial production, structure elucidation and bioconversion of sophorose lipids

TL;DR: A 17-hydroxyocta-decanoic acid at the C-1’ -position and acetate groups at the B-6’ and C-6' -positions of sophorose were found as substituents in the lactone and acidic forms of these lipids.
Journal ArticleDOI

Isolation of a bioemulsifier from Candida lipolytica.

TL;DR: The yeast Candida lipolytica produced an inducible extracellular emulsification activity when it was grown with a number of water-immiscible carbon substrates and this emulsifier, which the authors named liposan, effected stable oil-in-water emulsions with a variety of hydrocarbons.
Journal ArticleDOI

Chemical and Physical Characterization of Interfacial-Active Lipids from Rhodococcus erythropolis Grown on n-Alkanes.

TL;DR: Lipophilic compounds of the culture suspension containing Rhodococcus erythropolis DSM43215 had surfactant properties when the bacteria were cultivated with n-alkanes as the sole carbon source and the interfacial properties were stabile in solutions with a wide range of pH and ionic strength.
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