Journal ArticleDOI
Preparation of continuous beds for electrochromatography and reversed-phase liquid chromatography of low-molecular-mass compounds
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In this paper, the authors proposed a simpler and more cost-effective approach, not based on preformed beads but on continuous polymer beds synthesized in situ in the chromatographic tube.About:
This article is published in Journal of Chromatography A.The article was published on 1997-04-11. It has received 174 citations till now. The article focuses on the topics: Electrochromatography & Capillary electrochromatography.read more
Citations
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Journal ArticleDOI
Monolithic columns in high-performance liquid chromatography.
TL;DR: The actual and potential performance of monolithic columns are compared with those of packed columns andMonolithic columns have considerable advantages, which makes them most useful in many applications of liquid chromatography.
Journal ArticleDOI
Monolithic silica columns for high-efficiency chromatographic separations
Nobuo Tanaka,Hiroshi Kobayashi,Norio Ishizuka,Hiroyoshi Minakuchi,Kazuki Nakanishi,Ken Hosoya,Tohru Ikegami +6 more
TL;DR: Monolithic silica columns prepared from tetraalkoxysilane by a sol-gel method showed high efficiency and high permeability on the basis of the small-sized silica skeletons, large-sized through-pores, and resultingThrough-pore size/skeleton size ratios much larger than those found in a particle-packed column.
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Capillary columns with in situ formed porous monolithic packing for micro high-performance liquid chromatography and capillary electrochromatography
TL;DR: The efficiency of the monolithic columns in CEC was significantly higher than in mu-HPLC in the same way as observed with capillary columns having conventional particulate packing, due to the relaxation of band-broadening with electroosmotic flow (EOF) with respect to that with viscous flow.
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Monolithic stationary phases for liquid chromatography and capillary electrochromatography.
TL;DR: A monolithic stationary phase is the continuous unitary porous structure prepared by in situ polymerization or consolidation inside the column tubing and the surface is functionalized to convert it into a sorbent with the desired chromatographic binding properties.
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Monolithic porous polymer for on-chip solid-phase extraction and preconcentration prepared by photoinitiated in situ polymerization within a microfluidic device.
TL;DR: The function of the monolithic concentration device was first demonstrated using very dilute solutions of Coumarin 519 and the performance was then demonstrated with the enrichment of a hydrophobic tetrapeptide and also of green fluorescent protein for which an increase in concentration by a factor as high as 10(3) was achieved.
References
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High-performance electrophoresis : Elimination of electroendosmosis and solute adsorption
TL;DR: In this article, it was shown that electroendosmosis and adsorption of solutes onto the inside of the electrophoresis chamber should not occur in free high-performance electrophorensis when the narrow-bore tube is coated with a mono-molecular layer of polyacrylamide.
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High-performance liquid chromatography on continuous polymer beds
TL;DR: The experiments described in refs.
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Free zone electrophoresis
TL;DR: The free zone electrophoresis method described in this monograph can be used for the fractionation of small molecules, large molecules, and particles, and its versatility is illustrated in runs with inorganic ions, organic ions, nucleic acid bases, nucleosides, nucleotides, proteins,ucleic acids, subcellular particles, viruses, and erythrocytes.
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Miniaturisation in pressure and electroendosmotically driven liquid chromatography: Some theoretical considerations
John H. Knox,I. H. Grant +1 more
TL;DR: Electrochromatographic methods can provide a wide range of partitioning methods of extremely high plate efficiency, and will, in future, undoubtedly rival conventional HPLC.
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Open-tubular microcapillary liquid chromatography with electro-osmosis flow using a UV detector
TL;DR: In this article, the linear velocity of electro-osmosis in a capillary tube 30 ×90 cm x 30 −200 μm I.D. was measured, and the physical and geometrical parameters of EOSMosis and its use were discussed.