Proteoglycan-induced changes in T1ρ-relaxation of articular cartilage at 4T
Sarma V.S. Akella,Ravinder R. Regatte,Alexander J. Gougoutas,Arijitt Borthakur,Erik M. Shapiro,J. Bruce Kneeland,John S. Leigh,Ravinder Reddy +7 more
TLDR
Proteoglycan depletion‐induced changes in T1ρ (spin‐lattice relaxation in rotating frame) relaxation and dispersion in articular cartilage were studied at 4T and showed a strong correlation between changes in PG and T1RH.Abstract:
Proteoglycan (PG) depletion-induced changes in T1rho (spin-lattice relaxation in rotating frame) relaxation and dispersion in articular cartilage were studied at 4T. Using a spin-lock cluster pre-encoded fast spin echo sequence, T1rho maps of healthy bovine specimens and specimens that were subjected to PG depletion were computed at varying spin-lock frequencies. Sequential PG depletion was induced by trypsinization of cartilage for varying amounts of time. Results demonstrated that over 50% depletion of PG from bovine articular cartilage resulted in average T1rho increases from 110-170 ms. Regression analysis of the data showed a strong correlation (R2 = 0.987) between changes in PG and T1rho. T1rho values were highest at the superficial zone and decreased gradually in the middle zone and again showed an increasing trend in the region near the subchondral bone. The potentials of this method in detecting early degenerative changes of cartilage are discussed. Also, T(1rho)-dispersion changes as a function of PG depletion are described.read more
Citations
More filters
Journal ArticleDOI
Cartilage MRI T2 relaxation time mapping: overview and applications.
TL;DR: In vivo cartilage T2 mapping can improve understanding of arthritis, cartilage aging, and response of cartilage to exercise and help to develop new pharmaceuticals and surgical techniques for preserving cartilage.
Journal ArticleDOI
In vivo T1ρ and T2 mapping of articular cartilage in osteoarthritis of the knee using 3 T MRI
Xiaojuan Li,C. Benjamin Ma,Thomas M. Link,Darwin-Dean Castillo,Gabrielle Blumenkrantz,Jesus Lozano,Julio Carballido-Gamio,Michael D. Ries,Sharmila Majumdar +8 more
TL;DR: The ability to detect early cartilage degeneration prior to morphologic changes may allow us to critically monitor the course of OA and injury progression, and to evaluate the success of treatment to patients with early stages of Oa.
Journal ArticleDOI
Quantitative MRI of cartilage and bone: degenerative changes in osteoarthritis
TL;DR: The above methodologies show great promise for elucidating the pathophysiology of various tissues and identifying risk factors of osteoarthritis, for developing structure modifying drugs (DMOADs) and for combating osteOarthritis with new and better therapy.
Journal ArticleDOI
Articular Cartilage and Osteoarthiritis
Peter J. Neame,John A. Ogden +1 more
TL;DR: By reading, you can know the knowledge and things more, not only about what you get from people to people, but also about how to be successful in everything.
Journal ArticleDOI
T1rho relaxation mapping in human osteoarthritis (OA) cartilage: comparison of T1rho with T2.
TL;DR: To quantify the spin‐lattice relaxation time in the rotating frame (T1ρ) in various clinical grades of human osteoarthritis cartilage specimens obtained from total knee replacement surgery, and to correlate the T1ρ with OA disease progression and compare it with the transverse relaxation time (T2).
References
More filters
Journal ArticleDOI
A direct spectrophotometric microassay for sulfated glycosaminoglycans in cartilage cultures.
TL;DR: A rapid spectrophotometric procedure is described for the estimation of sulfated glycosaminoglycans in cartilage cultures that is substantially free from interference, is sensitive to less than 1 microgram (4 micrograms/ml) of chondroitin sulfate, and provides a simple alternative to the traditional methods for gly cosaminoglycan determinations.
Journal ArticleDOI
Gd‐DTPA2− as a measure of cartilage degradation
TL;DR: Equilibration of the tissue in Gd‐DTPA2‐ gives the opportunity to directly image (through T1, weighting) the concentration of GAG, a major and critically important macromolecule in cartilage, suggesting that this technique is clinically feasible.
Journal ArticleDOI
Protocol issues for delayed Gd(DTPA)(2-)-enhanced MRI (dGEMRIC) for clinical evaluation of articular cartilage.
Deborah Burstein,John H. Velyvis,Katherine T. Scott,Klaus W. Stock,Young-Jo Kim,Diego Jaramillo,Robert D. Boutin,Martha L. Gray +7 more
TL;DR: The factors that have been found to be important for the practical implementation of the delayed gadolinium‐enhanced MRI technique are described and in some cases of hypointensities in the subchondral patellar bone, decreased penetration of the contrast agent into cartilage from bone was found.
Journal ArticleDOI
Cartilage proteoglycans: structure and potential functions.
Peter J. Roughley,Eunice R. Lee +1 more
TL;DR: The largest in size and most abundant by weight is aggrecan, a proteoglycan that possesses over 100 chondroitin sulfate and keratan sulfate chains that provides cartilage with its osmotic properties, which give articular cartilage its ability to resist compressive loads.
Journal ArticleDOI
The correlation of fixed negative charge with glycosaminoglycan content of human articular cartilage
TL;DR: In the 25–60 age group neither the hexuronic acid nor the total hexosamine contents showed any correlation with the age of the subject, however, there was considerable local variation and variation between individuals.