Rapid method for the radioisotopic analysis of gaseous end products of anaerobic metabolism.
David R. Nelson,J. G. Zeikus +1 more
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A gas chromatographic procedure for the simultaneous analysis of 14C-labeled and unlabeled metabolic gases from microbial methanogenic systems is described in this article, where H2, CH4, and CO2 were separated within 2.5 min on a Carbosieve B column and were detected by thermal conductivity.Abstract:
A gas chromatographic procedure for the simultaneous analysis of 14C-labeled and unlabeled metabolic gases from microbial methanogenic systems is described. H2, CH4, and CO2 were separated within 2.5 min on a Carbosieve B column and were detected by thermal conductivity. Detector effluents were channeled into a gas proportional counter for measurement of radioactivity. This method was more rapid, sensitive, and convenient than gas chromatography-liquid scintillation techniques. The gas chromatography-gas proportional counting procedure was used to characterize the microbial decomposition of organic matter in anaerobic lake sediments and to monitor 14CH4 formation from H2 and 14CO2 by Methanosarcina barkeri.read more
Citations
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Book ChapterDOI
Physiological Ecology of Methanogens
TL;DR: Biological methanogenesis plays a major role in the carbon cycle on Earth and is the terminal step in carbon flow in many anaerobic habitats, including marine and freshwater sediments, marshes and swamps, flooded soils, bogs, geothermal habitats, and animal gastrointestinal tracts as discussed by the authors.
Journal ArticleDOI
The biology of methanogenic bacteria.
TL;DR: This article corrects the article on p. 517 in vol.
Journal ArticleDOI
Effect of sulfate on carbon and electron flow during microbial methanogenesis in freshwater sediments.
M R Winfrey,J G Zeikus +1 more
TL;DR: Results indicate that carbon and electron flow are altered when sulfate is added to sediments, and sulfate-reducing organisms appear to assume the role of methanogenic bacteria in sulfate-containing sediments by utilizing meethanogenic precursors.
Journal ArticleDOI
Non-aceticlastic methanogenesis from acetate: acetate oxidation by a thermophilic syntrophic coculture
Stephen H. Zinder,Markus Koch +1 more
TL;DR: Results support a mechanism for methanogenesis from acetate by the coculture in which acetate was oxidized to CO2 and H2 by one organism, while H2 was subsequently used by a second organism to reduce CO2 to CH4.
Journal ArticleDOI
Temperature limitation of methanogenesis in aquatic sediments.
J G Zeikus,M R Winfrey +1 more
TL;DR: The rate of sediment methanogenesis was shown to vary with respect to sediment site and depth, sampling date, in situ temperature, and number of methanogens, and correlated with increased sediment temperature during seasonal change.
References
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Journal ArticleDOI
Formation of Methane by Bacterial Extracts
TL;DR: The present communication is concerned with the formation of methane by cell-free extracts of &!
Journal ArticleDOI
The anaerobic mesophilic cellulolytic bacteria
TL;DR: Methods for pure culturing the cellulose bacteria have been developed and used for the isolation of numerous strains and some of the strains studied were probably successfully isolated, but the techniques were indirect and the criteria of separation could not be rigorous, leaving some doubt as to culture purity.
Journal ArticleDOI
An Improved Nonselective Culture Medium for Ruminal Bacteria and Its Use in Determining Diurnal Variation in Numbers of Bacteria in the Rumen
Marvin P. Bryant,I.M. Robinson +1 more
TL;DR: An improved rumen fluid agar medium was developed that permitted the growth of about double the numbers of bacteria from ruminal contents grown in the medium previously used, and allowed much larger numbers of colonies to be counted and isolated with greater ease from a given volume of medium and after longer incubation periods.
Book ChapterDOI
Microbial Formation of Methane
TL;DR: This chapter discusses the microbial formation of methane, a unique biological event, which is confined to a small group of bacteria, and the biochemistry of methane formation.
Related Papers (5)
Effect of sulfate on carbon and electron flow during microbial methanogenesis in freshwater sediments.
M R Winfrey,J G Zeikus +1 more