Journal ArticleDOI
Schistosomula (Schistosoma mansoni) obtained in vitro: Viability in culture and infectivity for mice
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This article is published in Transactions of The Royal Society of Tropical Medicine and Hygiene.The article was published on 1974-01-01. It has received 16 citations till now. The article focuses on the topics: Schistosoma mansoni & Infectivity.read more
Citations
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Journal ArticleDOI
Schistosoma mansoni: Defined system for stepwise transformation of cercaria to schistosomule in vitro
F. J. Ramalho-Pinto,G. Gazzinelli,R.E. Howells,T. A. Mota-santos,E.A. Figueiredo,J. Pellegrino +5 more
TL;DR: The resultant schistosomules are characterized by the depletion of their penetration gland contents, loss of tail, fluoride and water sensitivities, complement insensitivity, negative “Cercarien-hullen Reaktion,” and loss of the surface coat as demonstrated by periodic acid-Schiff (PAS) staining and electron microscope observations.
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Transformation of cercariae to schistosomula: a quantitative comparison of transformation techniques and of infectivity by different injection routes of the organisms produced.
E. R. James,M. G. Taylor +1 more
TL;DR: The peak for migration of schistosomula (following syringe transformation and intramuscular injection) through the lungs and the appearance of adult worms in the mesenteries was shown to be similar to cercarie administered percutaneously.
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Immunisation of sheep against Schistosoma mattheei using either irradiated cercariae or irradiated schistosomula.
TL;DR: The results indicate the possibility of making a live vaccine against ovine schistosomiasis and show that effective immunisation is not dependent on the presence of a mature worm infection or on cercarial penetration of the skin by the immunising infection.
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Schistosomiasis: progress and problems
George Y. Wu,Mohab H. Halim +1 more
TL;DR: Because both the schistosomes and the eggs utilize host metabolites, and because the host responses to the parasite are affected by its nutritional status, malnutrition may strongly affect both the parasite and the complex host-parasite relationship.
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Cultivation of schistosoma mansoni in vitro. iii. implantation of cultured worms into mouse mesenteric veins
Paul F. Basch,Richard Humbert +1 more
TL;DR: It is concluded that pairs once mature in vivo could regain the capacity for oviposition even after prolonged maintenance in vitro, but worms grown entirely in vitro to pairing may have missed some required stimulus which cannot be furnished later, even by an adequate animal host.
References
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In vitro cultivation of Schistosoma mansoni
TL;DR: The rate of growth and development of S. mansoni cultivated in vitro was assessed by comparison with the optimum rate of development in the white mouse and the contribution of culture techniques to the elucidation of schistosome physiology is discussed.
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Definition and collection in quantity of schistosomules of Schistosoma mansoni
TL;DR: The development of equipment and procedures is described for harvesting 15,000 or more schistosomules per collecting vessel, after they have penetrated dried, scraped, plucked rat abdominal skin.
Related Papers (5)
Schistosoma mansoni: simplified method for the production of schistosomules.
Resistance against Schistosoma mansoni induced by immunization of mice with cryopreserved schistosomula.
Quentin D. Bickle,E.R. James +1 more