Serpulina pilosicoli, waterbirds and water: potential sources of infection for humans and other animals
TLDR
Multilocus enzyme electrophoresis analysis of the isolates showed that they were genetically diverse, and none had identical electrophoretic profiles as those previously obtained from human beings, dogs, pigs and other avian species.Abstract:
Serpulina pilosicoli was isolated from 8 of 43 (19%) faecal specimens obtained from feral waterbirds sampled around a small lake at Perth Zoological Gardens, Western Australia, and from 3 of 7 (43%) samples of the lake water. The organism was only isolated from 1 of 204 (0·5%) samples from captive birds and animals in the zoological collection. Multilocus enzyme electrophoresis analysis of the isolates showed that they were genetically diverse, and none had identical electrophoretic profiles as those previously obtained from human beings, dogs, pigs and other avian species. To determine the survival time of S. pilosicoli in water, cells of strain 1648 were seeded into lake and tap water, and incubated at 4, 25 and 37°C. The organism could be recultured from lake water for up to 66 days at 4°C, and for 4 days at 25°C. A healthy human volunteer who drank water seeded with S. pilosicoli strain Wes B became colonized, and developed abdominal discomfort and headaches. Contamination of water by faeces may represent a source of S. pilosicoli infection for both humans and animals.read more
Citations
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Intestinal spirochete infections of chickens: a review of disease associations, epidemiology and control
C.P. Stephens,David J. Hampson +1 more
TL;DR: A recent survey of 136 layer and broiler breeder flocks in Australia revealed a high rate of infection (>40%) with intestinal spirochetes as mentioned in this paper, which was associated with wet litter problems and/or reduced egg production.
Journal ArticleDOI
Swine dysentery: aetiology, pathogenicity, determinants of transmission and the fight against the disease
Avelino Alvarez-Ordóñez,Francisco Javier Martínez-Lobo,Héctor Argüello,Ana Carvajal,Pedro Rubio +4 more
TL;DR: The knowledge available on the etiological agent of SD and its virulence traits are summarized, the determinants of SD transmission are reviewed, and the prophylactic and therapeutic approaches to fight against the disease are briefly described.
Journal ArticleDOI
Human intestinal spirochetosis: Brachyspira aalborgi and/or Brachyspira pilosicoli?
Andrew Mikosza,David J. Hampson +1 more
TL;DR: A review of recent developments in the study of HIS and the two spirochete species is outlined, and priorities for future research are identified.
Journal ArticleDOI
The Complete Genome Sequence of the Pathogenic Intestinal Spirochete Brachyspira pilosicoli and Comparison with Other Brachyspira Genomes
Phatthanaphong Wanchanthuek,Matthew I. Bellgard,Tom La,K. Ryan,Paula Moolhuijzen,Brett Chapman,Michael Black,D. Schibeci,Adam Hunter,Roberto A. Barrero,Nyree D. Phillips,David J. Hampson +11 more
TL;DR: The availability of the complete genome sequence of B. pilosicoli 95/1000 will facilitate functional genomics studies aimed at elucidating host-pathogen interactions and virulence.
Book ChapterDOI
Comparative Pathology of Bacterial Enteric Diseases of Swine
TL;DR: Enteric bacterial infections are among the most common and economically significant diseases affecting swine production worldwide and have been used as models to study the pathogenesis of similar diseases of human beings.
References
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Journal ArticleDOI
Serpulina pilosicoli sp. nov., the Agent of Porcine Intestinal Spirochetosis
Darren J. Trott,Thaddeus B. Stanton,Neil S. Jensen,Gerald E. Duhamel,John L. Johnson,David J. Hampson +5 more
TL;DR: DNA-DNA relative reassociation experiments in which the S1 nuclease method was used revealed that intestinal spirochete strain P43/6/78T was related to, but was genetically distinct from, both S. hyodysenteriae B78T and S. innocens B256T, and it is proposed that strain P 43/ 6/78 should be designated as the type strain of a new species, Serpulina pilosicoli.
Journal ArticleDOI
Autoclaved liquid medium for propagation of Treponema hyodysenteriae.
TL;DR: Three liquid media that differ slightly in composition but not in the method of preparation were developed for the propagation of TrePonema hyodysenteriae and Treponema innocens and supported better growth of T. hyodysteriae than did previously used liquid media.
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Recognition of two new species of intestinal spirochetes: Serpulina intermedia sp. nov. and Serpulina murdochii sp. nov.
Thad B. Stanton,Edith Fournié-Amazouz,D. Postic,Darren J. Trott,Patrick A. D. Grimont,G. Baranton,David J. Hampson,I. Saint Girons +7 more
TL;DR: On the basis of DNA-DNA hybridization data, nine intestinal spirochete strains were grouped into five genospecies and new Serpulina species were found, for which the names SerPulina intermedia sp.
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Isolation of Serpulina pilosicoli from Rectal Biopsy Specimens Showing Evidence of Intestinal Spirochetosis
TL;DR: The association between seeing spirochetes in biopsy specimens and isolating S. pilosicoli was statistically significant, clearly indicating that thisSpirochete is the agent of IS.
Journal ArticleDOI
Genetic characterisation of intestinal spirochaetes and their association with disease
J. I. Lee,David J. Hampson +1 more
TL;DR: Genetic relationships were assessed amongst 175 isolates of anaerobic intestinal spirochaetes, including 72 isolates from individuals living in different parts of the world, 102 from pigs and one from a dog, and amongst porcine isolates belonging to the genus Serpulina, a possible new species was identified.