Journal ArticleDOI
Somatic embryogenesis and plant regeneration in Sorghum bicolor (L.) Moench.
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TLDR
Callus induction, somatic embryogenesis and plant regeneration were obtained in two cultivars of Sorghum bicolor (L.) Moench and the importance of kinetin and dicamba on the induction of embryogenic potential is reported.Abstract:
Callus induction, somatic embryogenesis and plant regeneration were obtained in two cultivars of Sorghum bicolor (L.) Moench. Transverse thin cell layers from roots/epicotyls of 15-day-old seedlings or of regenerated plantlets were used. Callus response depended on the genotype, the size of transverse thin cell layers, the level at which transverse thin cell layers were excised on the epicotyl, the composition of growth substances and the number of in vitro regeneration cycles undergone by the donor plant. Somatic embryos were differentiated under a defined dark/light sequence, from epidermised compact calluses (i.e having already differentiated an epidermis), obtained directly with dicamba or from friable callus initiated with kinetin and 2,4 dichlorophenoxyacetic acid. The importance of kinetin and dicamba on the induction of embryogenic potential is reported.read more
Citations
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Journal ArticleDOI
Developmental and hormonal regulation of direct shoot organogenesis and somatic embryogenesis in sugarcane (Saccharum spp. interspecific hybrids) leaf culture.
Prakash Lakshmanan,R. Jason Geijskes,Lifang Wang,Adrian D. Elliott,Christopher P. L. Grof,Nils Berding,Grant R. Smith +6 more
TL;DR: Using a transverse thin cell layer culture system, some of the developmental and physiological constraints that limit high-frequency regeneration in sugarcane leaf tissue are identified and auxin, when added to the culture medium, reduced this spatial developmental constraint.
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High frequency shoot regeneration from Rhynchostylis gigantea (orchidaceae) using thin cell layers
TL;DR: Efficient micropropagation of direct production of shoots without passing through protocorm stage of orchid species can be achieved using the thin cell layer (TCL) method to Rhynchostylis gigantea.
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Manipulation of the morphogenetic pathways of Lilium longiflorum transverse thin cell layer explants by auxin and cytokinin
TL;DR: The establishment of different regeneration systems when explants are exposed to various growth regulators demonstrates that the choice of growth regulator combinations and concentrations are of significance in determining the morphogenetic response and plant regeneration capacity.
Journal ArticleDOI
Thin cell layer culture system in Lilium : Regeneration and transformation perspectives
TL;DR: The ability to manipulate and control organogenesis through a TCL system, coupled with repeatable, efficient transformation opens up the possibility of micropropagating members of this genus with new and available characteristics, producing superior plants of high quality.
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Efficient callus formation and plant regeneration of goosegrass [Eleusine indica (L.) Gaertn.].
TL;DR: Calli derived from the R-biotype of E. indica possessed a high resistance to trifluralin (dinitroaniline herbicide) and cross-resistance to a structurally non-related herbicide, amiprophosmethyl (phosphorothioamidate herbicide), as did the original resistant plants.
References
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TL;DR: It is reported that oligosaccharins derived from plant cell walls can induce tobacco expiants to form vegetative buds instead of flowers or callus, or flowers instead of vegetatives buds.
Journal ArticleDOI
Control of Morphogenesis in In Vitro Cultures
TL;DR: This work states that cell-cell interrelation, cell recognition, and cell division (mitosis and meiosis) in in vitro systems are driven by gene and epigenetic variabilities.
Journal ArticleDOI
Somatic embryogenesis from Sorghum bicolor leaves
TL;DR: It is reported that young leaf tissue of a cereal can express totipotency even to the extent of forming somatic embryos, however, the ability to respond is rapidly lost during leaf maturation, which can explain many difficulties faced in cereal tissue culture.
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