Sorbitol dehydrogenase, microorganism capable of producing the same and its production

TLDR: In this paper, the physicochemical properties of a sorbitol dehydrogenase were investigated, and it was shown that it has excellent substrate affinity and substrate specificity, and that it is possible to obtain microorganisms capable of producing the enzyme.

Abstract: PROBLEM TO BE SOLVED: To obtain a sorbitol dehydrogenase excellent in substrate affinity and substrate specificity, to obtain microorganisms capable of producing the enzyme, and to provide a method for producing the enzyme. SOLUTION: This sorbitol dehydrogenase has the physicochemical properties as follows: (1) action: dehydrogenically oxidizing D-sorbitol in the presence of NAD+ to form D-fructose; by the reaction reverse to the above, reducing D-fructose in the presence of NADH to form D-sorbitol and NAD+; (2) substrate specificity: when the Vmax/Km for D-sorbitol is assumed to be 100, the Vmax/Km for galactitol is about <=37, being about <=3 for L-iditol, and inert to D- arabitol, D-mannitol, xylitol and D-glucose; and (3) the Km for sorbitol is <=5.9 mM.