scispace - formally typeset
Journal ArticleDOI

Spectrophotometric determination of diosgenin in Dioscorea composita following thin-layer chromatography.

Gulliver López Sánchez, +2 more
- 01 Jan 1972 - 
- Vol. 97, Iss: 1161, pp 973-976
TLDR
A rapid and accurate method is described for the determination of diosgenin in Dioscorea composita roots by thin-layer chromatography and gas-liquid chromatography to evaluate statistically the accuracy of the method.
Abstract: 
A rapid and accurate method is described for the determination of diosgenin in Dioscorea composita roots. After acid hydrolysis and solvent extraction of the root, pure diosgenin is isolated by thin-layer chromatography, treated with sulphuric acid-methanol, and the resultant chromophore is measured by spectrophotometry.Similarly, gas-liquid chromatography is used to evaluate statistically the accuracy of the method.

read more

Citations
More filters
Journal ArticleDOI

Extraction of Diosgenin, a Bioactive Compound from Natural Source Dioscorea alata Var purpurae

Heena J Shah, +1 more
- 27 Aug 2012 - 
TL;DR: In this article, the diosgenin content was determined from tuber (fresh and dried) and callus of D. alata by acidic hydrolysis of the glycosides and extraction with an organic solvent, followed by HPTLC analysis.
Journal ArticleDOI

Diosgenin: An Updated Pharmacological Review and Therapeutic Perspectives

Prabhakar Semwal, +14 more
- 29 May 2022 - 
TL;DR: This review aims to provide an overview of the in vitro, in vivo, and clinical studies reporting the diosgenin's pharmacological effects and to discuss the safety issues.
Journal ArticleDOI

Recovery of Diosgenin from Dioscorea Rhizomes Using Aqueous Hydrotropic Solutions of Sodium Cumene Sulfonate

Sanjay P. Mishra, +1 more
- 26 Jun 2004 - 
TL;DR: In this article, aqueous solutions of aromatic hydrotropes were investigated for cell permeabilization and extraction of dioscin from dioscorea rhizomes, which was further hydrolyzed to diosgenin without significant decomposition to 3,5-diene.
Journal ArticleDOI

The Impact of Drought Stress and/or Nitrogen Fertilization in Some Medicinal Plants

Dea Baričevič, +1 more
- 25 Sep 2002 - 
TL;DR: The results of pot trials with fenugreek and with deadly nightshade are presented in this paper, showing that the maximal yield of tropane alkaloids (hyoscyamine: 54 mg/plant; scopolamine: 7 mg/plant) was achieved in plants grown under an optimal irrigation regime (35% depletion of available soil water) accompanied with a total...
Journal ArticleDOI

In vitro propagation and biosynthesis of steroidal sapogenins from various morphogenetic stages of Moringa oleifera Lam., and their antioxidant potential

Manas Mathur, +3 more
- 15 May 2014 - 
TL;DR: The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of bioactive constituents of this medicinal plant.
References
More filters
Book

Quantitative inorganic analysis

Gaston Charlot, +1 more
Related Papers (5)

The phenolic constituents of Prunus domestica. I.—The quantitative analysis of phenolic constituents

T. Swain, +1 more
- 01 Jan 1959 - 

A revised medium for rapid growth and bio assays with tobacco tissue cultures

Toshio Murashige, +1 more
- 01 Jul 1962 - 

A colorimetric method for the determination of phenols (and phenol derivatives) in urine

Otto Folin, +1 more
- 01 Sep 1915 - 

Natural Products: A Laboratory Guide

Raphael Ikan

Fungal diseases and their effect on phytochemical constituents of medicinally important Terminalia species in Bhadra Wildlife Sanctuary, Karnataka, India.

M. B. Shivanna, +1 more
- 01 Jan 2009 -