Open AccessJournal Article
Structure and biological activity of ascamycin, a new nucleoside antibiotic
Kiyoshi Isono,M Uramoto,H Osada,Makoto Ubukata,H Kusakabe,T Koyama,N Miyata,S K Sethi,James A. McCloskey +8 more
TLDR
Remarkable selective toxicity of ascamycin compared to the dealanyl derivative was accounted for on the basis of a dealanylating enzyme present in the envelope of sensitive bacteria.Abstract:
A newly isolated strain of Streptomyces sp. produces a new nucleoside antibiotic, ascamycin and the corresponding dealanyl derivative. The structure of ascamycin was determined to be 2-chloro-9-beta-[5-O-(N-L-alanyl)sulfamoyl-D-ribofuranosyl]-adenine. Remarkable selective toxicity of ascamycin compared to the dealanyl derivative was accounted for on the basis of a dealanylating enzyme present in the envelope of sensitive bacteria. After dealanylation, it becomes permeable to cell membrane.read more
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Inhibition of the D-alanine:D-alanyl carrier protein ligase from Bacillus subtilis increases the bacterium's susceptibility to antibiotics that target the cell wall.
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Trapping interactions between catalytic domains and carrier proteins of modular biosynthetic enzymes with chemical probes
TL;DR: The design and implementation of the chemical tools that have proven to be useful in biochemical and biophysical studies of these natural product biosynthetic enzymes are described.
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Enhanced Production of the Fluorinated Nucleoside Antibiotic Nucleocidin by a rifR-Resistant Mutant of Streptomyces calvus IFO13200
TL;DR: To activate the dormant secondary metabolism of S. calvus, the effect of an rpoB mutation that was induced by ultraviolet light irradiation showed remarkably improved antibiotic activity, which was extracted by n-butanol and identified by Electron-Spray-Ionization Mass Spectrometry.