Journal ArticleDOI
The Covalent Structure of Beef Heart Myoglobin
TLDR
The covalent structure of beef myoglobin is being investigated through different experimental approaches through tryptic and chymotryptic digestions and the complete amino acid sequence of these peptides has been established.Abstract:
The covalent structure of beef myoglobin is being investigated through different experimental approaches. The globin was cleaved by cyanogen bromide. The resulting fragments were fractionated and submitted to tryptic and chymotryptic digestions. The complete amino acid sequence of these peptides has been established. From the overlaps in sequence between chymotryptic and tryptic peptides, it is possible to place most of these peptides of 153 residues of beef myoglobin in unique sequence. The remaining residues have been ordered on the basis of the apparent homology in sequence between beef and horse myoglobins. The horse protein differs from that of beef in 18 positions. All of the amino acid replacements, except three, were confined to amino acid interchange caused by the change of one base in the coding triplet leaving 135 of 153 positions in the sequence invariant in the two proteins. The relations of these data to other is discussed.read more
Citations
More filters
Journal ArticleDOI
Myoglobin chemistry and meat color.
Surendranath P. Suman,P. Joseph +1 more
TL;DR: An overview of the current research in meat color and how the findings are applied in the meat industry is provided to help engineer innovative processing strategies to minimize meat discoloration-induced revenue loss to the agricultural economy.
Journal ArticleDOI
The dependence of immunological cross-reactivity upon sequence resemblance among lysozymes. I. Micro-complement fixation studies.
Ellen M. Prager,Allan C. Wilson +1 more
TL;DR: A correlation was observed between degree of immunological cross-reactivity and degree of amino acid sequence similarity, with the limitation that proteins differing from each other by 40% or more in sequence exhibited no cross- reactivity in micro-complement fixation tests.
Journal ArticleDOI
Darwinian evolution in the genealogy of haemoglobin.
TL;DR: Frequencies of mutations between reconstructed ancestor and descendant sequences of codons for metazoan globin chains show that natural selection guided protein evolution.
Journal ArticleDOI
The dependence of immunological cross-reactivity upon sequence resemblance among lysozymes. II. Comparison of precipitin and micro-complement fixation results.
Ellen M. Prager,Allan C. Wilson +1 more
TL;DR: It seems to be a general rule that native proteins differing by more than 30 to 40% in sequence fail to cross-react in direct precipitin or complement fixation tests.
Journal ArticleDOI
Molecular Evolution of Myoglobin and the Fossil Record: a Phylogenetic Synthesis
TL;DR: An ancestral myoglobin chain has been deduced by comparing the differences in the amino acid sequences of eighteen living species and assessing from the fossil evidence the probable times of divergence of their ancestors.
References
More filters
Journal ArticleDOI
Automatic recording apparatus for use in the chromatography of amino acids.
Journal ArticleDOI
A Protein Sequenator
TL;DR: The protein sequenator is an instrument for the automatic determination of amino acid sequences in proteins and peptides that operates on the principle of the phenylisothiocyanate degradation scheme and has been applied to the whole molecule of apomyoglobin from the humpback whale.
Journal ArticleDOI
Chemical Determination of Tryptophan in Proteins
J. R. Spies,D. C. Chambers +1 more