Journal ArticleDOI
The effect of iron and iron chelators on the in-vitro block to development of the mouse preimplantation embryo: BAT6 a new medium for improved culture of mouse embryos in vitro
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TLDR
A new medium, BAT6, is described for the optimal in-vitro culture of mouse embryos after an adverse effect of iron was found and both the rate of embryonic development and the proportion of embryos reaching the blastocyst stage approached levels found in vivo.Abstract:
The effect of iron and iron chelators on the development of the mouse embryo in vitro from the 1-cell stage to the blastocyst has been investigated. An adverse effect of iron was found. The high affinity iron chelator, desferal, also blocked development, whilst transferrin (whether as apoprotein or saturated with iron), DETAPAC and EDTA promoted development. The addition of transferrin permitted development to the blastocyst stage of embryos from stains normally exhibiting the 2-cell block. Under such circumstances both the rate of embryonic development and the proportion of embryos reaching the blastocyst stage approached levels found in vivo. Based on these results, a new medium, BAT6, is described for the optimal in-vitro culture of mouse embryos.read more
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Journal ArticleDOI
Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings
TL;DR: It is now common to add antioxidant compounds to culture media, but maintaining the pro-oxidant-antioxidant equilibrium in embryos through such supplementation is a complex problem.
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Detection of reactive oxygen species (ROS) and apoptosis in human fragmented embryos.
TL;DR: It is concluded that there is a direct relationship between increased H2O2 concentration and apoptosis, and that further studies should be undertaken to confirm these findings.
Journal ArticleDOI
Radical solutions and cultural problems: Could free oxygen radicals be responsible for the impaired development of preimplantation mammalian embryos in vitro?
TL;DR: Much evidence reviewed here implicates free oxygen radicals (FORs) in the process of arrest and the arrest or delay of most embryos in vitro can be reduced or prevented experimentally by addition of metal chelators to limit hydroxy radical formation and lipid hydroperoxidation.
Increased generation of reactive oxygen species in embryo cultured in vitro
TL;DR: In this article, the level of H2O2 in individual embryos using a fluorimetric method was measured, and it was shown that the level increased with the exposure to visible light.