Journal ArticleDOI
The reactions of isolated mucopolysaccharides to several histochemical tests.
TLDR
It is concluded, therefore, that the use of the PAS technic for the histochemical demonstration of acid mucopolysaccharides is misleading, for many im...Abstract:
The reaction to three histochemical tests of preparations of hyaluronic acid, chondroitin sulfate, heparin, the acidic mucopolysaccharides from cornea, gastric mucin, and dentine, and also of the neutral mucopolysaccharide from gastric mucin was studied. To 1% aqueous solutions of the acid mucopolysaccharides, equal volumes of 1% casein solution were added; drops of the resulting solutions were placed on slides and dried at 37 °C. The films were then fixed in acetic-alcohol (1:9). The technics employed were the periodic acid-Schiff (PAS) test, the metachromatic reaction and the Hale test. The relative acidity of the preparations was demonstrated by staining in dilute aqueous methylene blue at pH 3-6. With the exception of the preparation from dentine, the acid mucopolysaccharides stained only weakly with PAS; the neutral mucopolysaccharide stained strongly. It is concluded, therefore, that the use of the PAS technic for the histochemical demonstration of acid mucopolysaccharides is misleading, for many im...read more
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Diamine methods for differentialing mucosubstances histochemically.
TL;DR: A mixture of N,N-dimethyl-rn-phenylenediamine (HCI)2 with the para isonter at pH 3.5-4.0 stains acid mucosubstautces selectively as mentioned in this paper.
Journal ArticleDOI
The special value of methods that color both acidic and vicinal hydroxyl groups in the histochemical study of mucins. with revised directions for the colloidal iron stain, the use of alcian blue g8x and their combinations with the periodic acid‐schiff reaction*
Journal ArticleDOI
Metachromasia; chemical theory and histochemical use.
Maxwell Schubert,David Hamerman +1 more
TL;DR: The purpose of the present review is to bring together togethem’ n’ecesst ideas from several separated fields of study miot always withini the i mmimsmediate s’anige of i midivi(lual workers i nsterested its nssetachronimnut ic stainui nsg.
Journal ArticleDOI
Glycoproteins and diabetes.
TL;DR: Present knowledge of glycoproteins structure and metabolism is reviewed to attempt to assess their role in the pathogenesis of the diabetic lesions and the quantitative importance of their biosynthesis as a pathway of glucose metabolism.
References
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A microchemical reaction resulting in the staining of polysaccharide structures in fixed tissue preparations
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The organization of ground substance and basement membrane and its significance in tissue injury disease and growth.
Journal ArticleDOI
Histochemical Demonstration of Acid Polysaccharides in Animal Tissues
TL;DR: The objective is to establish an experimental procedure and show direct AFM measurements that unequivocally can be assigned as the carrier and removal of polysaccharides from the animal body.
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The mucopolysaccharides of bovine cornea.
TL;DR: The mucopolysaccharides isolated from various mesodermal tissues have, in several instances, been shown to consist of complex mixtures, and not of single compounds as was believed formerly, so it was thought advisable to reinvestigate the mucopoly Saccharide fractions of the cornea.