The term affinity chromatography seems to have been first used by Cuatrecasas et al. (1968); however, the principles involved have long been known in the field of immunology. In fact, as early as 1936, Landsteiner and van der Scheer used insolubilized haptens for the isolation of the corresponding antibodies. Since then numerous examples of antibody purification by use of immunosorbents have been reported (Silman and Katchalski, 1966).

The Use of Affinity Chromatography in the Purification of Steroid-Binding Proteins

Biologically active proteins and polypeptides can be coupled by various means to cellulose, starch and cross-linked polysaccharide gels such as ‘Sephadex’. The use of cyanogen halides for this purpose gives a high yield of bound polypeptide or protein which retains a substantial part of its activity.

Chemical Coupling of Peptides and Proteins to Polysaccharides by Means of Cyanogen Halides

In this article, Cuatrecasas, Wilchek, and Anfinsen explain the general principles and potential applications of "affinity
 chromatography," a protein purification technique that is indispensable to modern biological research.

https://www.pnas.org/content/pnas/61/2/636.full.pdf

Selective enzyme purification by affinity chromatography

WE have reported a method for transforming polysaccharides into reactive derivatives useful for coupling proteins to carbohydrates1 which consists in treating the polysaccharide with cyanogen halide. Imino carbonic acid esters are probably formed and these react with the primary amino groups of proteins and other substances. This method of activation and coupling is gentle and therefore particularly useful for the production of immunosorbents and insoluble enzymes.

The Use of Affinity Chromatography in the Purification of Steroid-Binding Proteins

References

Chemical Coupling of Peptides and Proteins to Polysaccharides by Means of Cyanogen Halides

Selective enzyme purification by affinity chromatography

Chemical Coupling of Proteins to Agarose

Solid-phase radioimmunoassay of estradiol-17 beta

Radioimmunoassay of proteins with the use of Sephadex-coupled antibodies

Related Papers (5)

Affinity chromatography. New approaches for the preparation of spacer containing derivatives and for specific isolation of peptides.

Practical protein chromatography

Affinity chromatography : methods and protocols

The Use of Affinity Chromatography for the Specific Purification of Antibodies and Antigens

Agarose derivatives for purification of protein by affinity chromatography.