Showing papers in "Biochimica et Biophysica Acta in 1966"

TL;DR: With a Stokes radius measured by the chromatographic method and a sedimentation coefficient determined by density gradient centrifugation, reasonable estimates for both the molecular weight and the frictional ratio (f/f0) of a macromolecule are available.

TL;DR: The result of experiments indicate that the synthesis of nitrate reductase is induced by nitrate and repressed by ammonium, and no effect in vitro of ammonium could be found.

TL;DR: It is suggested that the first step in N 2 reduction is a two-electron reduction leading to a non-dissociable intermediate at the oxidation level of diimide.

TL;DR: Kinetic and genetic evidences are presented to show that, in addition to specific amino acid permeases, Saccharomyces cerevisiae has a general amino acids permease which catalyzes the transport of basic and neutral amino acids, but most probably not that of proline.

TL;DR: A conclusion has been drawn that the enzyme isolated from a product of Aspergillus oryzae is a denatured DNA-(probably single stranded- DNA-)specific nuclease.

TL;DR: The structure of human fibrinogen and the specificity of thrombin is discussed, with a focus on the A-, AP- and Y-peptides.

TL;DR: This finding strongly suggests that the anucleolate Xenopus is a deletion mutant, and supports the hypothesis that ribosomal cistrons are located at the nucleolar organizer region.

TL;DR: Spinach chloroplasts were fragmented by incubation with digitonin and the resulting chlorophyll-containing particles separated by differential centrifugation, and particles with the high chl a/chl b ratios appear to be representative of System 1 or the “long wavelength” system of photosynthesis, whereas the 1000 × g and 10 000 × g fractions seem to be enriched in particles representative of system 2.

TL;DR: The major fatty acid constituents in the total lipids of the diatoms examined were palmitoleic, palmitic, eicosapentaenoic, and eICosatetraenoic acids ; small amounts of hexadecadienoic, hexadecatrienoic

TL;DR: The data presented in this paper show that the fixed-angle rotor offers from 5 to 10-fold greater resolution than the swinging-bucket rotor and that it has a capacity per tube of at least 10 times that of the swingside rotor.

TL;DR: Functionally inactive copper present in the enzyme preparation, added Cu(II) and Fe(III) salts and mitochrome, a modification product of cytochrome c oxidase, are not reduced by phenazine methosulphate, under the conditions of the titration.

TL;DR: In the presence of E. coli endogenous mRNA instead of polyuridylic acid, acetylphenylalanyl-sRNA, surprisingly, is not used for protein synthesis and is instead partially used as donor of phenylalanine in internal positions during polypeptide-chain formation.

TL;DR: Suitable assay conditions are described for the NADPH-dependent oxidative desaturation of stearyl-CoA by rat-liver microsomes and the electron-transfer mechanisms associated with these microsomal reactions are discussed.

TL;DR: A quantitative analysis of the fluorescence induction in isolated chloroplasts is presented, finding that, besides Q, a second electron carrier, P, is involved, reacting in a consecutive dark step: Q and P are present in a ratio 1:1, the concentration of each being 1:70 chlorophyll.

TL;DR: It is inferred that nuclei incubated in the absence of ammonium sulphate may synthesize ribosomal RNA, and that the action of ammonia sulphate is to induce the synthesis of an RNA more DNA-like in its base composition.

TL;DR: Plasma membranes have been isolated from rat liver and the ATPase and (Na+-K+)-ATPase and 5′-nucleotidase activities have been studied and the relation between membrane structure and enzymic function is discussed.

TL;DR: Centrifugation of rat-liver ribosomal plus rapidly labelled RNA through a sucrose gradient at pH 6 produces a distribution of radioactivity with a peak at S > 30, which is reproducible and can be altered by administration of triamcinolone, insulin or menaphthone to the animal.

TL;DR: In this paper, a Na +, K + -dependent ATPase was obtained from calf-heart muscle by succesive treatments with deoxycholate and NaI and the optimal pH was 7.2; the K m for ATP for the Na+,K+ -dependent and Mg 2+-dependent enzyme activities were 2.4·10 −4 M and 5.0·10−5 M, respectively.

TL;DR: Evidence is taken as support for the view that some of the components of the acetylcholine-activated permeability system at the junctional regions of the innervated membrane of the electroplax are proteins.

TL;DR: The amino acid compositions of plasmodium actin and muscle actin are strikingly similar and appear to be similar to those of muscle G-actin.

TL;DR: The nitrate reductase of tobacco cells grown in suspension culture on a chemically defined medium is induced by nitrate, and the enzyme decays in cells grown into nitrate starvation, and it is not formed in cells growing on casein hydrolysate as the sole nitrogen source.

TL;DR: The peroxidase-thiocyanate-H 2 O 2 antimicrobial system in inhibited by catalase as well as by a number of low-molecular-weight substance which may be present in the growth media, or in biological fluids is considered.

TL;DR: It is tentatively proposed that the affinity of the inhibitory site(s) of phosph ofructokinase for ATP is affected by hydrogen ion concentration and that aging causes a change of conformation of the enzyme, thus blocking the control mechanism of phosphofructokin enzyme.

TL;DR: The efficient protection against thiol reagent inhibition by substrate supports a supposition that this -SH group forms a covalent bond with substrate during the catalytic reaction.

TL;DR: It is assumed that repression of the synthesis of alcohol dehydrogenase, malate dehydrogen enzyme, isocitrate lyase and malate synthase is not caused by metabolites but by glucose itself, glucose 6-phos-phate, or perhaps by changes in the concentrations of adenosine 5'-triphosphate or -diphosphate.

TL;DR: Cell membranes were prepared from turkey erythrocytes by fragmentation into small particles or larger pieces which led to a loss of responsiveness to epinephrine and could be restored by prior incubation with a sulfhydryl reagent such as mercaptoethanol.

TL;DR: Inhibition studies indicate that the l -arabinose permease system of Escherichia coli B/r may also mediate the transport of d -xylose, d -frucose, and d -galactose.