Tissue culture propagation method for dendrobium gibsonii lindl

TLDR: In this paper, a tissue culture propagation method for dendrobium gibsonii lindl was proposed, where newly-sprouted tender buds of healthy plants were used as explants and disinfected, and the disinfected explants were put into an MS basic culture medium to be induced to obtain sterile test tube seedlings.

Abstract: The invention provides a tissue culture propagation method for dendrobium gibsonii lindl. The tissue culture propagation method comprises the following steps: (1) taking newly-sprouted tender buds of healthy plants of the dendrobium gibsonii lindl as explants and disinfecting; (2) putting the disinfected explants into an MS basic culture medium to be induced to obtain sterile test tube seedlings; and (3) putting the sterile test tube seedlings into an MS propagation culture medium to perform rapid propagation culture of the test tube seedlings to obtain cluster buds. With the adoption of the culture method provided by the invention, the growth coefficient of the obtained cluster buds of the dendrobium gibsonii lindl can be increased by 20-28 times, high-quality dendrobium gibsonii lindl germchits with a high survival rate can be provided in short time, and a large-scale seedling growing problem of the dendrobium gibsonii lindl is effectively solved.