Tissue explants used for enhancement of herpesvirus isolations.
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TLDR
There is evidence to suggest that the explant method is more sensitive than the use of tissue homogenates for recovery of other viruses as well.Abstract:
Herpesvirus recovery from normal and experimentally infected simian tissues prepared into 10 to 20% homogenates was compared with that obtained by explanting these same tissues. The explant procedure was by far the more sensitive of the two methods. As a result, 8 herpesvirus isolations were obtained by conventional virus isolation procedures in contrast to 74 isolates when explants were made from these same tissue sources. There is also evidence to suggest that the explant method is more sensitive than the use of tissue homogenates for recovery of other viruses as well.read more
Citations
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Latent infection of sensory ganglia with herpes simplex virus: efficacy of immunization
TL;DR: Testing the efficacy of active immunization in preventing latent infection of local sensory ganglia that follows inoculation of superficial epithelial surfaces with herpes simplex virus found substantial but not complete protection was observed.
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Activation of latent herpesvirus hominis in explants of rabbit trigeminal ganglia: the influence of immune serum.
TL;DR: If the ganglia were explanted in the presence of immune serum, the virus recovery rate was at least twice lower as compared to the virus activation in explants kept in the absence of immune plasma serum.
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In vitro white spot syndrome virus (WSSV) replication in explants of the heart of freshwater crab, Paratelphusa hydrodomous
TL;DR: The results indicate that the heart explants of P. hydrodomous would be a good alternative to whole animals for production of WSSV.
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Clinical, virological, and pathological features of herpesvirus SA8 infection in conventional and gnotobiotic infant baboons (Papio cynocephalus).
TL;DR: There was a significant difference in total white blood counts (WBC), with higher values in conventional vs. gnotobiotic infants, the absolute number of lymphocytes was not different, and the lower number of WBCs apparently was due to fewer polymorphonuclear leukocytes in the g notobiotic baboons.
Journal ArticleDOI
Experimental herpesvirus infection of baboons (Papio cynocephalus) and African green monkeys (Cercopithecus aethiops) and recovery of virus by tissue explants
TL;DR: Adult African green monkeys and young baboons were inoculated with a baboon-derived herpesvirus (0430) which is antigenically related to herpesv virus SA8 and recovery of virus from explants was superior to recovery from tissue homogenates using routine methods.
References
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Isolation of a cytopathogenic agent from human adenoids undergoing spontaneous degeneration in tissue culture.
TL;DR: An unidentified, possibly new, tissue culture cytopathogenic agent has been isolated repeatedly from human adenoids undergoing spontaneous degeneration in tissue culture, and it is tentatively proposed to designate the agent as theadenoid degeneration agent.
Journal ArticleDOI
Isolation of Measles Virus from Cell Cultures of Brain from a Patient with Subacute Sclerosing Panencephalitis
TL;DR: In vitro propagation of a patient with subacute sclerosing panencephalitis and cocultivation of the brain cells with a line of green-monkey-kidney cells offers direct evidence that measles virus has a a role in the etiology of the disease.
Journal ArticleDOI
Serial propagation in vitro of agents producing inclusion bodies derived from varicella and herpes zoster.
TL;DR: From the eruptive lesions of two cases of herpes zoster, cytopathogenic agents of a similar nature have been isolated and have been propagated serially in cultures of human tissue.
Journal ArticleDOI
Isolation of measles virus from brain cell cultures of two patients with subacute sclerosing panencephalitis
Luiz Horta-Barbosa,David A. Fuccillo,W. T. London,J. T. Jabbour,Wolfgang Zeman,John L. Sever +5 more
TL;DR: Measles virus was isolated from brain cell tissue cultures derived from two SSPE patients that proved to contain intracellular measles antigen which was not released in the fluid phase.
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